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  • KD/KO Validated

PTPRA Polyclonal antibody

PTPRA Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 13079-1-AP

Synonyms

LRP, HPTPalpha, HPTPA, HLPR, HEPTP


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Tested Applications

Positive WB detected inA431 cells, rat liver tissue, MCF-7 cells, SKOV-3 cells
Positive IP detected inA431 cells
Positive IHC detected inhuman lung cancer tissue, human urothelial carcinoma tissue, mouse heart tissue, mouse kidney tissue, rat kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13079-1-AP targets PTPRA in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PTPRA fusion protein Ag3760 相同性解析による交差性が予測される生物種
Full Name protein tyrosine phosphatase, receptor type, A
Calculated molecular weight 793 aa, 91 kDa
Observed molecular weight90-105 kDa, 140 kDa
GenBank accession numberBC027308
Gene symbol PTPRA
Gene ID (NCBI) 5786
RRIDAB_2269055
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PTPRA antibody 13079-1-APDownload protocol
IHC protocol for PTPRA antibody 13079-1-APDownload protocol
IF protocol for PTPRA antibody 13079-1-APDownload protocol
IP protocol for PTPRA antibody 13079-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oncol Lett

PTPRA facilitates cancer growth and migration via the TNF-α-mediated PTPRA-NF-κB pathway in MCF-7 breast cancer cells.

Authors - Canfeng Lin
  • KO Validated
humanWB,IF,IP

Oncogene

Glucose-mediated N-glycosylation of RPTPα affects its subcellular localization and Src activation

Authors - Jiayu Fang
mouseWB

Sci Signal

Clustering of phosphatase RPTPα promotes Src signaling and the arthritogenic action of synovial fibroblasts

Authors - Sho Sendo
humanWB

EBioMedicine

The circRNA circPTPRA suppresses epithelial-mesenchymal transitioning and metastasis of NSCLC cells by sponging miR-96-5p.

Authors - Siliang Wei
  • KD Validated