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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

LRP2BP Polyclonal antibody

LRP2BP Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25783-1-AP

Synonyms

KIAA1325, LRP2 binding protein, LRP2BP, Megalin binding protein, MegBP

Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using LRP2BP Polyclonal antibody (25783-1-AP)

    WB analysis of A549 using 25783-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 25783-1-AP (LRP2BP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat lung tissue using LRP2BP Polyclonal antibody (25783-1-AP)

    WB analysis of rat lung using 25783-1-AP

    rat lung tissue were subjected to SDS PAGE followed by western blot with 25783-1-AP (LRP2BP Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

  • Western Blot (WB) analysis of Jurkat cells using LRP2BP Polyclonal antibody (25783-1-AP)

    WB analysis of Jurkat using 25783-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 25783-1-AP (LRP2BP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human cerebellum tissue using LRP2BP Polyclonal antibody (25783-1-AP)

    IHC staining of human cerebellum using 25783-1-AP

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 25783-1-AP (LRP2BP antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human cerebellum tissue using LRP2BP Polyclonal antibody (25783-1-AP)

    IHC staining of human cerebellum using 25783-1-AP

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 25783-1-AP (LRP2BP antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

Positive WB detected inA549 cells, Jurkat cells, rat lung tissue
Positive IHC detected inhuman cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25783-1-AP is LRP2BP Fusion Protein expressed in E. coli.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LRP2BP fusion protein Ag22779 相同性解析による交差性が予測される生物種
Full Name LRP2 binding protein
Calculated molecular weight 347 aa, 40 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC152444
Gene symbol LRP2BP
Gene ID (NCBI) 55805
RRIDAB_2880238
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for LRP2BP antibody 25783-1-APDownload protocol
IHC protocol for LRP2BP antibody 25783-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols