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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

LRRC18 Polyclonal antibody

LRRC18 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24571-1-AP

Synonyms

LRRC18, UNQ933, UNQ9338, VKGE9338

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using LRRC18 Polyclonal antibody (24571-1-AP)

    WB analysis of mouse testis using 24571-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 24571-1-AP (LRRC18 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse testis tissue using LRRC18 Polyclonal antibody (24571-1-AP)

    IP experiment of mouse testis using 24571-1-AP

    IP result of anti-LRRC18 (IP:24571-1-AP, 3ug; Detection:24571-1-AP 1:500) with mouse testis tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human testis tissue using LRRC18 Polyclonal antibody (24571-1-AP)

    IHC staining of human testis using 24571-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24571-1-AP (LRRC18 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using LRRC18 Polyclonal antibody (24571-1-AP)

    IHC staining of human testis using 24571-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24571-1-AP (LRRC18 Antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

Positive WB detected inmouse testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24571-1-AP is LRRC18 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LRRC18 fusion protein Ag20996 相同性解析による交差性が予測される生物種
Full Name leucine rich repeat containing 18
Calculated molecular weight 261 aa, 30 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC036722
Gene symbol LRRC18
Gene ID (NCBI) 474354
RRIDAB_2879614
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Leucine rich repeat containing protein 18 ( LRRC18 ) encodes a 261 amino-acids protein (29.6 kDa), which contains 7 leucine rich repeat and belongs to LRR superfamily. The function of protein is still non-known, but it may be involved in the regulation of spermatogenesis and sperm maturation. The protein localizes in cytoplasm and express in some male reproduction tissues.

Protocols

Product Specific Protocols
WB protocol for LRRC18 antibody 24571-1-APDownload protocol
IHC protocol for LRRC18 antibody 24571-1-APDownload protocol
IP protocol for LRRC18 antibody 24571-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols