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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

LRRC20 Polyclonal antibody

LRRC20 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 25562-1-AP

Synonyms

UNQ2429/PRO4989, Leucine-rich repeat-containing protein 20

Validation Data Gallery

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  • Western Blot (WB) analysis of NIH/3T3 cells using LRRC20 Polyclonal antibody (25562-1-AP)

    WB analysis of NIH/3T3 using 25562-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 25562-1-AP (LRRC20 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

  • Western Blot (WB) analysis of Y79 cells using LRRC20 Polyclonal antibody (25562-1-AP)

    WB analysis of Y79 using 25562-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 25562-1-AP (LRRC20 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using LRRC20 Polyclonal antibody (25562-1-AP)

    IHC staining of human stomach cancer using 25562-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 25562-1-AP (LRRC20 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using LRRC20 Polyclonal antibody (25562-1-AP)

    IHC staining of human testis using 25562-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25562-1-AP (LRRC20 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using LRRC20 Polyclonal antibody (25562-1-AP)

    IHC staining of human stomach cancer using 25562-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 25562-1-AP (LRRC20 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using LRRC20 Polyclonal antibody (25562-1-AP)

    IHC staining of human stomach cancer using 25562-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 25562-1-AP (LRRC20 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using LRRC20 Polyclonal antibody (25562-1-AP)

    IHC staining of human colon using 25562-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25562-1-AP (LRRC20 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using LRRC20 Polyclonal antibody (25562-1-AP)

    IHC staining of human colon using 25562-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25562-1-AP (LRRC20 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using LRRC20 Polyclonal antibody (25562-1-AP)

    IHC staining of human stomach cancer using 25562-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 25562-1-AP (LRRC20 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using LRRC20 Polyclonal antibody (25562-1-AP)

    IHC staining of human testis using 25562-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25562-1-AP (LRRC20 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using LRRC20 Polyclonal antibody (25562-1-AP)

    IF Staining of A431 using 25562-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using LRRC20 antibody (25562-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inNIH/3T3 cells, Y79 cells
Positive IHC detected inhuman stomach cancer tissue, human colon tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25562-1-AP targets LRRC20 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LRRC20 fusion protein Ag22119 相同性解析による交差性が予測される生物種
Full Name leucine rich repeat containing 20
Calculated molecular weight 184 aa, 21 kDa
Observed molecular weight 21-25 kDa
GenBank accession numberBC024001
Gene symbol LRRC20
Gene ID (NCBI) 55222
RRIDAB_2880132
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for LRRC20 antibody 25562-1-APDownload protocol
IHC protocol for LRRC20 antibody 25562-1-APDownload protocol
IF protocol for LRRC20 antibody 25562-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols