Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis of mouse liver using 23217-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 23217-1-AP (LRRC47 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of Neuro-2a using 23217-1-AP
Neuro-2a cells were subjected to SDS PAGE followed by western blot with 23217-1-AP (LRRC47 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of rat liver using 23217-1-AP
rat liver tissue were subjected to SDS PAGE followed by western blot with 23217-1-AP (LRRC47 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 23217-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 23217-1-AP (LRRC47 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of Neuro-2a using 23217-1-AP
Neuro-2a cells were subjected to SDS PAGE followed by western blot with 23217-1-AP (LRRC47 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of L02 using 23217-1-AP
L02 cells were subjected to SDS PAGE followed by western blot with 23217-1-AP (LRRC47 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human pancreas using 23217-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas slide using 23217-1-AP (LRRC47 Antibody) at dilution of 1:50.
IHC staining of human pancreas using 23217-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas slide using 23217-1-AP (LRRC47 Antibody) at dilution of 1:50.
IF Staining of L02 using 23217-1-AP
Immunofluorescent analysis of L02 cells using 23217-1-AP (LRRC47 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

LRRC47 Polyclonal antibody

LRRC47 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 23217-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Synonyms

Leucine-rich repeat-containing protein 47, KIAA1185

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (3)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse liver tissue, L02 cells, Neuro-2a cells, NIH/3T3 cells, rat liver tissue
Positive IHC detected in	human pancreas tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	L02 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 3 publications below
IHC	See 1 publications below
CoIP	See 1 publications below

Product Information

23217-1-AP targets LRRC47 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	LRRC47 fusion protein Ag19736 相同性解析による交差性が予測される生物種
Full Name	leucine rich repeat containing 47
Calculated molecular weight	583 aa, 63 kDa
Observed molecular weight	63 kDa
GenBank accession number	BC031301
Gene Symbol	LRRC47
Gene ID (NCBI)	57470
RRID	AB_2879234
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen Affinity purified
UNIPROT ID	Q8N1G4
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

LRRC47 enables RNA binding activity. It is predicted to be involved in phenylalanyl-tRNA aminoacylation.

Protocols

Product Specific Protocols
WB protocol for LRRC47 antibody 23217-1-AP	Download protocol
IHC protocol for LRRC47 antibody 23217-1-AP	Download protocol
IF protocol for LRRC47 antibody 23217-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nature Structural basis for the final steps of human 40S ribosome maturation. Authors - Michael Ameismeier KD Validated View Article
human	WB	bioRxiv LARP1 senses free ribosomes to coordinate supply and demand of ribosomal proteins Authors - James A Saba KD Validated View Article
human	WB,CoIP,IHC	Cell Rep The C9ORF72 repeat expansion alters neurodevelopment Authors - Eric Hendricks View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of mouse liver using 23217-1-AP

WB analysis of Neuro-2a using 23217-1-AP

WB analysis of rat liver using 23217-1-AP

WB analysis of NIH/3T3 using 23217-1-AP

WB analysis of Neuro-2a using 23217-1-AP

WB analysis of L02 using 23217-1-AP

IHC staining of human pancreas using 23217-1-AP