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LRRC47 Polyclonal antibody
LRRC47 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 23217-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse liver tissue, L02 cells, Neuro-2a cells, NIH/3T3 cells, rat liver tissue |
Positive IHC detected in | human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | L02 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 2 publications below |
WB | See 3 publications below |
IHC | See 1 publications below |
CoIP | See 1 publications below |
Product Information
23217-1-AP targets LRRC47 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | LRRC47 fusion protein Ag19736 相同性解析による交差性が予測される生物種 |
Full Name | leucine rich repeat containing 47 |
Calculated molecular weight | 583 aa, 63 kDa |
Observed molecular weight | 63 kDa |
GenBank accession number | BC031301 |
Gene symbol | LRRC47 |
Gene ID (NCBI) | 57470 |
RRID | AB_2879234 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen Affinity purified |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
LRRC47 enables RNA binding activity. It is predicted to be involved in phenylalanyl-tRNA aminoacylation.
Protocols
Product Specific Protocols | |
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WB protocol for LRRC47 antibody 23217-1-AP | Download protocol |
IHC protocol for LRRC47 antibody 23217-1-AP | Download protocol |
IF protocol for LRRC47 antibody 23217-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nature Structural basis for the final steps of human 40S ribosome maturation.
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bioRxiv LARP1 senses free ribosomes to coordinate supply and demand of ribosomal proteins
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