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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

LRRC4C Polyclonal antibody

LRRC4C Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13845-1-AP

Synonyms

KIAA1580, LRRC4C, Netrin G1 ligand, NGL 1, NGL1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of Y79 cells using LRRC4C Polyclonal antibody (13845-1-AP)

    WB analysis of Y79 using 13845-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 13845-1-AP (LRRC4C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using LRRC4C Polyclonal antibody (13845-1-AP)

    IHC staining of human gliomas using 13845-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas using 13845-1-AP (LRRC4C antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using LRRC4C Polyclonal antibody (13845-1-AP)

    IHC staining of human gliomas using 13845-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas using 13845-1-AP (LRRC4C antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

Positive WB detected inY79 cells
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 2 publications below

Product Information

13845-1-AP targets LRRC4C in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LRRC4C fusion protein Ag4811 相同性解析による交差性が予測される生物種
Full Name leucine rich repeat containing 4C
Calculated molecular weight 640 aa, 72 kDa
Observed molecular weight 72 kDa
GenBank accession numberBC041374
Gene symbol LRRC4C
Gene ID (NCBI) 57689
RRIDAB_2137623
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for LRRC4C antibody 13845-1-APDownload protocol
IHC protocol for LRRC4C antibody 13845-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Cell Rep Med

Single-cell sequencing identifies differentiation-related markers for molecular classification and recurrence prediction of PitNET

Authors - Qilin Zhang
View Article
humanIF

Mol Med

LRRC4 functions as a neuron-protective role in experimental autoimmune encephalomyelitis.

Authors - Yan Zhang
View Article