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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

LRRC8D Polyclonal antibody

LRRC8D Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 11537-1-AP

Synonyms

Volume-regulated anion channel subunit LRRC8D, LRRC5, Leucine-rich repeat-containing protein 8D, Leucine-rich repeat-containing protein 5, HsLRRC8D

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using LRRC8D Polyclonal antibody (11537-1-AP)

    WB analysis of HEK-293 using 11537-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 11537-1-AP (LRRC8D antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using LRRC8D Polyclonal antibody (11537-1-AP)

    WB analysis of mouse brain using 11537-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 11537-1-AP (LRRC8D antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human brain tissue using LRRC8D Polyclonal antibody (11537-1-AP)

    IHC staining of human brain using 11537-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 11537-1-AP (LRRC8D antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human brain tissue using LRRC8D Polyclonal antibody (11537-1-AP)

    IHC staining of human brain using 11537-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 11537-1-AP (LRRC8D antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using LRRC8D Polyclonal antibody (11537-1-AP)

    IF Staining of HeLa using 11537-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 11537-1-AP (LRRC8D antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inmouse brain tissue, HEK-293 cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11537-1-AP targets LRRC8D in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LRRC8D fusion protein Ag2138 相同性解析による交差性が予測される生物種
Full Name leucine rich repeat containing 8 family, member D
Calculated molecular weight 858 aa, 98 kDa
Observed molecular weight 98-120 kDa
GenBank accession numberBC024159
Gene symbol LRRC8D
Gene ID (NCBI) 55144
RRIDAB_2137767
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LRRC8D (or LRRC5L) gene encodes a 98-kDa multi-pass membrane protein leucine-rich repeat-containing protein 8D, which contains 13 LRRs (leucine-rich repeats). The molecular function of this protein is so far not well understood. Recently, LRRC8D gene was found to be up-regulated after IL-2 deprivation in immortalised Interleukin-2(IL-2)-dependent human T cells, indicating a possible involvement of LRRC8D in cancer development. This LRRC8D antibody can't cross-recognize LRRC8A.

Protocols

Product Specific Protocols
WB protocol for LRRC8D antibody 11537-1-APDownload protocol
IHC protocol for LRRC8D antibody 11537-1-APDownload protocol
IF protocol for LRRC8D antibody 11537-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Cell Physiol Biochem

Cell Volume Regulation in the Epidermis.

Authors - Magdalena Jahn
View Article
humanWB

Cell Genom

Protein interaction studies in human induced neurons indicate convergent biology underlying autism spectrum disorders

Authors - Greta Pintacuda
View Article