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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

LRRTM1 Polyclonal antibody

LRRTM1 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 14288-1-AP

Synonyms

UNQ675/PRO1309, Leucine-rich repeat transmembrane neuronal protein 1

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using LRRTM1 Polyclonal antibody (14288-1-AP)

    WB analysis of HEK-293 using 14288-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 14288-1-AP (LRRTM1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using LRRTM1 Polyclonal antibody (14288-1-AP)

    WB analysis of HeLa using 14288-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14288-1-AP (LRRTM1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using LRRTM1 Polyclonal antibody (14288-1-AP)

    WB analysis of mouse brain using 14288-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 14288-1-AP (LRRTM1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using LRRTM1 Polyclonal antibody (14288-1-AP)

    IHC staining of human testis using 14288-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 14288-1-AP (LRRTM1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using LRRTM1 Polyclonal antibody (14288-1-AP)

    IHC staining of human testis using 14288-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 14288-1-AP (LRRTM1 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using LRRTM1 Polyclonal antibody (14288-1-AP)

    IHC staining of human brain using 14288-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 14288-1-AP (LRRTM1 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using LRRTM1 Polyclonal antibody (14288-1-AP)

    IHC staining of human brain using 14288-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 14288-1-AP (LRRTM1 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using LRRTM1 Polyclonal antibody (14288-1-AP)

    IF Staining of mouse brain using 14288-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 14288-1-AP (LRRTM1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, mouse brain tissue
Positive IHC detected inhuman testis tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14288-1-AP targets LRRTM1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LRRTM1 fusion protein Ag5602 相同性解析による交差性が予測される生物種
Full Name leucine rich repeat transmembrane neuronal 1
Calculated molecular weight 59 kDa
Observed molecular weight 59 kDa
GenBank accession numberBC045113
Gene symbol LRRTM1
Gene ID (NCBI) 347730
RRIDAB_2138065
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for LRRTM1 antibody 14288-1-APDownload protocol
IHC protocol for LRRTM1 antibody 14288-1-APDownload protocol
IF protocol for LRRTM1 antibody 14288-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols