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- KD/KO Validated
Perilipin 5 Polyclonal antibody
Perilipin 5 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, pig, mouse, rat and More (1)
Applications
WB, IHC, IF, IP, ELISA
Conjugate
Unconjugated
Cat no : 26951-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | MCF-7 cells, pig heart tissue, mouse liver tissue, mouse heart tissue, rat heart tissue |
| Positive IHC detected in | human liver tissue, mouse heart tissue, mouse brown adipose tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 19 publications below |
| IHC | See 2 publications below |
| IF | See 3 publications below |
| IP | See 1 publications below |
Product Information
26951-1-AP targets Perilipin 5 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, pig, mouse, rat samples.
| Tested Reactivity | human, pig, mouse, rat |
| Cited Reactivity | human, mouse, rat, goat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Perilipin 5 fusion protein Ag25272 相同性解析による交差性が予測される生物種 |
| Full Name | lipid storage droplet protein 5 |
| Calculated molecular weight | 463 aa, 51 kDa |
| Observed molecular weight | 51-55 kDa |
| GenBank accession number | BC131524 |
| Gene symbol | Perilipin 5 |
| Gene ID (NCBI) | 440503 |
| RRID | AB_2880699 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Perilipin 5 (also known as LSDP5/OXPAT) is a lipid droplet (LD) coat protein that promotes association of LDs with mitochondria and is mainly present in tissues with a high fat-oxidative capacity, such as heart, skeletal muscles and brown adipose tissue. It plays an important role in the regulation of cardiac lipid storage and function.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Perilipin 5 antibody 26951-1-AP | Download protocol |
| IHC protocol for Perilipin 5 antibody 26951-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Mol Cell Biol Knockdown of hepatocyte Perilipin-3 mitigates hepatic steatosis and steatohepatitis caused by hepatocyte CGI-58 deletion in mice | ||
EMBO Rep miR-183 and miR-96 orchestrate both glucose and fat utilization in skeletal muscle. | ||
Int J Mol Sci Effects of Simvastatin on Lipid Metabolism in Wild-Type Mice and Mice with Muscle PGC-1α Overexpression. | ||
Int Immunopharmacol Perilipin 5 protects against oxygen-glucose deprivation/reoxygenation-elicited neuronal damage by inhibiting oxidative stress and inflammatory injury via the Akt-GSK-3β-Nrf2 pathway. | ||
Hum Cell Aryl hydrocarbon receptor promotes lipid droplet biogenesis and metabolic shift in respiratory Club cells. | ||
Biochem Biophys Res Commun Perilipin 5 ameliorates high-glucose-induced podocyte injury via Akt/GSK-3β/Nrf2-mediated suppression of apoptosis, oxidative stress, and inflammation. |
