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  • Featured Product
  • KD/KO Validated

LSG1 Polyclonal antibody

LSG1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Cat no : 17750-1-AP

Synonyms

hLsg1, Large subunit GTPase 1 homolog, LSG1

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using LSG1 Polyclonal antibody (17750-1-AP)

    WB analysis of HeLa using 17750-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17750-1-AP (LSG1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using LSG1 Polyclonal antibody (17750-1-AP)

    WB analysis of HeLa using 17750-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17750-1-AP (LSG1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using LSG1 Polyclonal antibody (17750-1-AP)

    IP experiment of HeLa using 17750-1-AP

    IP result of anti-LSG1 (IP:17750-1-AP, 4ug; Detection:17750-1-AP 1:500) with HeLa cells lysate 1400 ug.

  • Immunohistochemistry (IHC) staining of human testis tissue using LSG1 Polyclonal antibody (17750-1-AP)

    IHC staining of human testis using 17750-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 17750-1-AP (LSG1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using LSG1 Polyclonal antibody (17750-1-AP)

    IHC staining of human testis using 17750-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 17750-1-AP (LSG1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using LSG1 Polyclonal antibody (17750-1-AP)

    IHC staining of human spleen using 17750-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 17750-1-AP (LSG1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using LSG1 Polyclonal antibody (17750-1-AP)

    IHC staining of human spleen using 17750-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 17750-1-AP (LSG1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using LSG1 Polyclonal antibody (17750-1-AP)

    IHC staining of human kidney using 17750-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 17750-1-AP (LSG1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using LSG1 Polyclonal antibody (17750-1-AP)

    IHC staining of human kidney using 17750-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 17750-1-AP (LSG1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using LSG1 Polyclonal antibody (17750-1-AP)

    IHC staining of human placenta using 17750-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 17750-1-AP (LSG1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using LSG1 Polyclonal antibody (17750-1-AP)

    IHC staining of human placenta using 17750-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 17750-1-AP (LSG1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using LSG1 Polyclonal antibody (17750-1-AP)

    IHC staining of human lung using 17750-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 17750-1-AP (LSG1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using LSG1 Polyclonal antibody (17750-1-AP)

    IHC staining of human lung using 17750-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 17750-1-AP (LSG1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using LSG1 Polyclonal antibody (17750-1-AP)

    IHC staining of human ovary using 17750-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 17750-1-AP (LSG1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using LSG1 Polyclonal antibody (17750-1-AP)

    IHC staining of human ovary using 17750-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 17750-1-AP (LSG1 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

Positive WB detected inHeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman testis tissue, human kidney tissue, human lung tissue, human ovary tissue, human placenta tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17750-1-AP targets LSG1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LSG1 fusion protein Ag12016 相同性解析による交差性が予測される生物種
Full Name large subunit GTPase 1 homolog (S. cerevisiae)
Calculated molecular weight 658 aa, 75 kDa
Observed molecular weight 75 kDa
GenBank accession numberBC068500
Gene symbol LSG1
Gene ID (NCBI) 55341
RRIDAB_2281443
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for LSG1 antibody 17750-1-APDownload protocol
IHC protocol for LSG1 antibody 17750-1-APDownload protocol
IP protocol for LSG1 antibody 17750-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Aging Cell

Inhibition of the 60S ribosome biogenesis GTPase LSG1 causes endoplasmic reticular disruption and cellular senescence.

Authors - Asimina Pantazi
  • KD Validated
View Article
humanWB

Mol Cell Biol

The Ribosome Assembly Factor LSG1 Interacts with Vesicle-Associated Membrane Protein-Associated Proteins (VAPs)

Authors - Putri Sutjita
View Article