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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

LSM1 Polyclonal antibody

LSM1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10259-1-AP

Synonyms

Cancer associated Sm like, CASM, LSM1, Small nuclear ribonuclear CaSm, YJL124C

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using LSM1 Polyclonal antibody (10259-1-AP)

    WB analysis of HeLa using 10259-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10259-1-AP (LSM1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using LSM1 Polyclonal antibody (10259-1-AP)

    WB analysis of mouse kidney using 10259-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10259-1-AP (LSM1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using LSM1 Polyclonal antibody (10259-1-AP)

    IP experiment of HeLa using 10259-1-AP

    IP result of anti-LSM1 (IP:10259-1-AP, 4ug; Detection:10259-1-AP 1:300) with HeLa cells lysate 2000ug.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using LSM1 Polyclonal antibody (10259-1-AP)

    IHC staining of human prostate cancer using 10259-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10259-1-AP (LSM1 antibody) at dilution of 1:100 (under 10x lens).

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Tested Applications

Positive WB detected inHeLa cells, mouse kidney tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10259-1-AP targets LSM1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LSM1 fusion protein Ag0232 相同性解析による交差性が予測される生物種
Full Name LSM1 homolog, U6 small nuclear RNA associated (S. cerevisiae)
Calculated molecular weight 15 kDa
Observed molecular weight 18-20 kDa
GenBank accession numberBC001767
Gene symbol LSM1
Gene ID (NCBI) 27257
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Lsm1 is a component of the Lsm1-7 complex involved in cytoplasmic mRNA degradation. Lsm1 is a key subunit that determines the RNA-binding properties of this complex. The normal RNA-binding activity of this complex is crucial for mRNA decay and 3'-end protection in vivo and requires the intact Sm-domain of Lsm1. LSM1 localizes to cytoplasmic processing bodies (P bodies) involved in mRNA storage, processing, regulation, and degradation and is required for P-body formation. LSM1 proteins facilitate the formation of U4/U6 RNA duplexes in vitro and may play a role in U4/U6 snRNP formation.