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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LSM12 Monoclonal antibody (67029-1-Ig)

    WB analysis using 67029-1-Ig (same clone as 67029-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67029-1-Ig (LSM12 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67029-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using LSM12 Monoclonal antibody (67029-1-Ig)

    IHC staining of human colon cancer using 67029-1-Ig (same clone as 67029-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67029-1-Ig (LSM12 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67029-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using LSM12 Monoclonal antibody (67029-1-Ig)

    IHC staining of human colon cancer using 67029-1-Ig (same clone as 67029-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67029-1-Ig (LSM12 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67029-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using LSM12 Monoclonal antibody (67029-1-Ig)

    IHC staining of human colon cancer using 67029-1-Ig (same clone as 67029-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67029-1-Ig (LSM12 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67029-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using LSM12 Monoclonal antibody (67029-1-Ig)

    IHC staining of human colon cancer using 67029-1-Ig (same clone as 67029-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67029-1-Ig (LSM12 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67029-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using LSM12 Monoclonal antibody (67029-1-Ig)

    IHC staining of human colon cancer using 67029-1-Ig (same clone as 67029-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67029-1-Ig (LSM12 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67029-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using LSM12 Monoclonal antibody (67029-1-Ig)

    FC experiment of HeLa using 67029-1-Ig (same clone as 67029-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug LSM12 Monoclonal antibody (67029-1-Ig, Clone:2E3G3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67029-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

LSM12 Monoclonal antibody, PBS Only

LSM12 Monoclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA
Cat No. 67029-1-PBS
Clone No.2E3G3

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), Indirect ELISA

Synonyms

Protein LSM12, 2E3G3

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67029-1-PBS targets LSM12 in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen LSM12 fusion protein Ag28782 相同性解析による交差性が予測される生物種
Full Name LSM12 homolog (S. cerevisiae)
Observed molecular weight 22 kDa
GenBank accession numberBC044587
Gene Symbol LSM12
Gene ID (NCBI) 124801
RRIDAB_2882344
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ3MHD2
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.