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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

LSM14A Polyclonal antibody, PBS Only

LSM14A Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 18336-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LSM14A Polyclonal antibody (18336-1-AP)

    WB analysis using 18336-1-AP (same clone as 18336-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 18336-1-AP (LSM14A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18336-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using LSM14A Polyclonal antibody (18336-1-AP)

    WB analysis of HeLa using 18336-1-AP (same clone as 18336-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 18336-1-AP (LSM14A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18336-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using LSM14A Polyclonal antibody (18336-1-AP)

    WB analysis of A549 using 18336-1-AP (same clone as 18336-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 18336-1-AP (LSM14A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18336-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-3 cells using LSM14A Polyclonal antibody (18336-1-AP)

    WB analysis of PC-3 using 18336-1-AP (same clone as 18336-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 18336-1-AP (LSM14A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18336-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using LSM14A Polyclonal antibody (18336-1-AP)

    IHC staining of human testis using 18336-1-AP (same clone as 18336-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 18336-1-AP (LSM14A antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 18336-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using LSM14A Polyclonal antibody (18336-1-AP)

    IHC staining of human testis using 18336-1-AP (same clone as 18336-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 18336-1-AP (LSM14A antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 18336-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using LSM14A Polyclonal antibody (18336-1-AP)

    IF Staining of HepG2 using 18336-1-AP (same clone as 18336-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 18336-1-AP (LSM14A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18336-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

18336-1-PBS targets LSM14A in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LSM14A fusion protein Ag12997 相同性解析による交差性が予測される生物種
Full Name LSM14A, SCD6 homolog A (S. cerevisiae)
Calculated molecular weight 463 aa, 51 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC016842
Gene symbol LSM14A
Gene ID (NCBI) 26065
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

LSm14A was originally discovered to be a component of the P-bodies, it is a member of the LSm family of proteins, which have been shown to be involved in RNA processing events. The N terminus of LSm14A is a conserved LSm domain, whereas the C terminus contains two domains called "DFDF box" and "FDF_TFG box", respectively.