• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

LSR Polyclonal antibody

LSR Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18216-1-AP

Synonyms

ILDR3, LISCH, LISCH7, LSR

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HT-29 cells using LSR Polyclonal antibody (18216-1-AP)

    WB analysis of HT-29 using 18216-1-AP

    HT-29 cells (LSR+) and HeLa cells (LSR-) were subjected to SDS PAGE followed by western blot with 18216-1-AP (LSR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using LSR Polyclonal antibody (18216-1-AP)

    WB analysis using 18216-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18216-1-AP (LSR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse stomach tissue using LSR Polyclonal antibody (18216-1-AP)

    IHC staining of mouse stomach using 18216-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 18216-1-AP (LSR antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse stomach tissue using LSR Polyclonal antibody (18216-1-AP)

    IHC staining of mouse stomach using 18216-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 18216-1-AP (LSR antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using LSR Polyclonal antibody (18216-1-AP)

    IHC staining of mouse liver using 18216-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 18216-1-AP (LSR antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using LSR Polyclonal antibody (18216-1-AP)

    IHC staining of mouse liver using 18216-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 18216-1-AP (LSR antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

☆対象製品が30%オフ！組換えタンパク質&免染用試薬 >>> キャンペーン実施中！

Tested Applications

Positive WB detected inHT-29 cells, HepG2 cells, mouse liver tissue, rat liver tissue
Positive IHC detected inmouse stomach tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

18216-1-AP targets LSR in WB, IHC, ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Reactivity human, rat, mouse
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LSR fusion protein Ag12961 相同性解析による交差性が予測される生物種
Full Name lipolysis stimulated lipoprotein receptor
Calculated molecular weight 71 kDa
Observed molecular weight 60-70 kDa, 56 kDa
GenBank accession numberBC004381
Gene symbol LSR
Gene ID (NCBI) 51599
RRIDAB_2250137
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LSR (lipolysis stimulated lipoprotein receptor) is a lipoprotein receptor primarily expressed in the liver and activated by free fatty acids (PMID: 10224102). This remnant receptor binds both apoB- and apoE-containing lipoproteins and displays greatest affinity for TG-rich lipoproteins. Distribution of LSR causes embryonic lethality in mice, indicating that the expression of LSR is critical for liver and embryonic development (PMID: 15265030). Investigations show that LSR is a multimer consisting of α subunit (68 kDa) and β subunit (56 kDa) associated through disulfide bridges (PMID: 10224102; 19188430; 18644789).

Protocols

Product Specific Protocols
WB protocol for LSR antibody 18216-1-APDownload protocol
IHC protocol for LSR antibody 18216-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Biosens Bioelectron

A multiple mixed TiO2 mesocrystal junction based PEC-colorimetric immunoassay for specific recognition of lipolysis stimulated lipoprotein receptor.

Authors - Yanjie Chen
View Article
mouseWB

J Hazard Mater

Multi-omics analyses reveal the mechanisms of Arsenic-induced male reproductive toxicity in mice.

Authors - Zijun Peng
View Article