LSS Polyclonal antibody

LSS Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 13715-1-AP

Synonyms

Oxidosqualene--lanosterol cyclase, OSC, Lanosterol synthase, hOSC, EC:5.4.99.7


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Tested Applications

Positive WB detected inHT-1376 cells, PC-3 cells, HeLa cells, HepG2 cells, mouse testis tissue, human brain tissue, mouse pancreas tissue, mouse liver tissue, SH-SY5Y cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman liver tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13715-1-AP targets LSS in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, rabbit
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LSS fusion protein Ag4655 相同性解析による交差性が予測される生物種
Full Name lanosterol synthase (2,3-oxidosqualene-lanosterol cyclase)
Calculated molecular weight 732 aa, 83 kDa
Observed molecular weight 70-75 kDa
GenBank accession numberBC035638
Gene symbol LSS
Gene ID (NCBI) 4047
RRIDAB_10597096
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LSS, also named as OSC, is lanosterol synthase which catalyzes the cyclization of (S)-2,3 oxidosqualene to lanosterol, a reaction that forms the sterol nucleus. LSS gene has three isoforms with MW 74 kDa and 82-85 kDa. This antibody can recognize all these three isoforms.

Protocols

Product Specific Protocols
WB protocol for LSS antibody 13715-1-APDownload protocol
IHC protocol for LSS antibody 13715-1-APDownload protocol
IF protocol for LSS antibody 13715-1-APDownload protocol
IP protocol for LSS antibody 13715-1-APDownload protocol
FC protocol for LSS antibody 13715-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Neuron

Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory.

Authors - Xiaohui Li
humanWB

J Invest Dermatol

Bi-allelic Variants in LSS Cause Palmoplantar Keratoderma-Congenital Alopecia Syndrome Type 2.

Authors - Fang Yang
humanWB,IHC

PLoS Genet

Metabolic and pathologic profiles of human LSS deficiency recapitulated in mice.

Authors - Yoichi Wada
ratWB

J Biol Chem

The Endoplasmic Reticulum COPII-Vesicle Transport Machinery Coordinates Cellular Lipid Secretion and Cholesterol Biosynthesis.

Authors - Lee G D Fryer
humanWB, IHC

Phytomedicine

Hibiscus syriacus L. cultivated in callus culture exerts cytotoxicity in colorectal cancer via Notch signaling-mediated cholesterol biosynthesis suppression.

Authors - Xing Yue Xu
humanWB

J Biol Chem

The antiviral enzyme viperin inhibits cholesterol biosynthesis.

Authors - Timothy J Grunkemeyer