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LST1 Polyclonal antibody

LST1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 21361-1-AP

Synonyms

Protein B144, LST 1, Leukocyte-specific transcript 1 protein, D6S49E, B144

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LST1 Polyclonal antibody (21361-1-AP)

    WB analysis using 21361-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21361-1-AP (LST1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of THP-1 cells using LST1 Polyclonal antibody (21361-1-AP)

    WB analysis of THP-1 using 21361-1-AP

    WB result of LST1 antibody (21361-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LST1 transfected THP-1 cells.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using LST1 Polyclonal antibody (21361-1-AP)

    IHC staining of mouse brain using 21361-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21361-1-AP (LST1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using LST1 Polyclonal antibody (21361-1-AP)

    IHC staining of mouse brain using 21361-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21361-1-AP (LST1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using LST1 Polyclonal antibody (21361-1-AP)

    IF Staining of HeLa using 21361-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using LST1 antibody (21361-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inTHP-1 cells, mouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

21361-1-AP targets LST1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LST1 fusion protein Ag15817 相同性解析による交差性が予測される生物種
Full Name leukocyte specific transcript 1
Calculated molecular weight 97 aa, 11 kDa
Observed molecular weight10-15 kDa
GenBank accession numberBC103856
Gene symbol LST1
Gene ID (NCBI) 7940
RRIDAB_2918071
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LST1 (Leukocyte Specific Transcript 1), also known as B144, is a small adaptor protein expressed in leukocytes of myeloid lineage. LST1 splice variants (LST1/A-LST1/N) have been detected in various cell types, and thought to affect mainly LST1 gene expression and splicing, are associated with inflammatory conditions(PMID: 10706707, PMID: 33986741). The molecular weight of LSTI protein may vary between different lysates.

Protocols

Product Specific Protocols
WB protocol for LST1 antibody 21361-1-APDownload protocol
IHC protocol for LST1 antibody 21361-1-APDownload protocol
IF protocol for LST1 antibody 21361-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Braz J Otorhinolaryngol

Significance of leukocyte-specific transcript 1 levels in nasal mucosal tissue to predict recurrence of nasal polyps

Authors - Junyi Zhang
View Article