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KD/KO Validated

LTBR Polyclonal antibody

Name: LTBR Polyclonal antibody
Brand: Proteintech
SKU: 20331-1-AP

LTBR Polyclonal Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 20331-1-AP

Synonyms

TNF RIII, TNF R III, Lymphotoxin-beta receptor, Lymphotoxin beta receptor, LT BETA R

Validation Data Gallery

Filter:

AllWBIHCFC (Intra)

WB analysis of HeLa using 20331-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 20331-1-AP (LTBR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 20331-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 20331-1-AP (LTBR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 20331-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 20331-1-AP (LTBR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 20331-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 20331-1-AP (LTBR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human hepatocirrhosis using 20331-1-AP
Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 20331-1-AP (LTBR antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human hepatocirrhosis using 20331-1-AP
Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 20331-1-AP (LTBR antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human hepatocirrhosis using 20331-1-AP
Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 20331-1-AP (LTBR antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human hepatocirrhosis using 20331-1-AP
Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 20331-1-AP (LTBR antibody) at dilution of 1:50 (under 40x lens).
FC experiment of HeLa using 20331-1-AP
1x10^6 HeLa cells were intracellularly stained with 0.4 ug LTBR Polyclonal antibody (20331-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2) (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Cat No. 20331-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, mouse brain tissue
Positive IHC detected in	human hepatocirrhosis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive FC (Intra) detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:5000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 3 publications below
IHC	See 2 publications below
IF	See 4 publications below

Product Information

20331-1-AP targets LTBR in WB, IHC, IF, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	LTBR fusion protein Ag14065 相同性解析による交差性が予測される生物種
Full Name	lymphotoxin beta receptor (TNFR superfamily, member 3)
Calculated molecular weight	435 aa, 47 kDa
Observed molecular weight	47-60 kDa
GenBank accession number	BC026262
Gene symbol	LTBR
Gene ID (NCBI)	4055
RRID	AB_10694276
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for LTBR antibody 20331-1-AP	Download protocol
IHC protocol for LTBR antibody 20331-1-AP	Download protocol
FC protocol for LTBR antibody 20331-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IF	Mol Ther Activation of NF-κB-Inducing Kinase in Islet β Cells Causes β Cell Failure and Diabetes. Authors - Xinzhi Li View Article
human	WB	J Immunol SNX17 Affects T Cell Activation by Regulating TCR and Integrin Recycling. Authors - Douglas G Osborne View Article
human	IHC,IF,WB	Am J Pathol Lymphotoxins Promote the Progression of Human Lymphatic Malformation by Enhancing Lymphatic Endothelial Cell Proliferation. Authors - Jie-Gang Yang KD Validated View Article
		Mol Med Rep Lymphotoxin β receptor activation promotes mRNA expression of RelA and pro-inflammatory cytokines TNFα and IL-1β in bladder cancer cells. Authors - Mo Shen View Article
mouse	IF	Theranostics Islet α-cell Inflammation Induced By NF-κB inducing kinase (NIK) Leads to Hypoglycemia, Pancreatitis, Growth Retardation, and Postnatal Death in Mice. Authors - Xinzhi Li View Article
human	IHC,IF	J Reprod Immunol A LIGHT-HVEM/LTβR axis contributes to the fibrosis of intrauterine adhesion Authors - Ayitila Abudukeyoumu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

LTBR Polyclonal antibody

LTBR Polyclonal Antibody for WB, IHC, FC (Intra), ELISA

Validation Data Gallery

WB analysis of HeLa using 20331-1-AP

WB analysis of mouse brain using 20331-1-AP

WB analysis of mouse brain using 20331-1-AP

WB analysis of mouse brain using 20331-1-AP

IHC staining of human hepatocirrhosis using 20331-1-AP

IHC staining of human hepatocirrhosis using 20331-1-AP

IHC staining of human hepatocirrhosis using 20331-1-AP

IHC staining of human hepatocirrhosis using 20331-1-AP

FC experiment of HeLa using 20331-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Activation of NF-κB-Inducing Kinase in Islet β Cells Causes β Cell Failure and Diabetes.

SNX17 Affects T Cell Activation by Regulating TCR and Integrin Recycling.

Lymphotoxins Promote the Progression of Human Lymphatic Malformation by Enhancing Lymphatic Endothelial Cell Proliferation.

Lymphotoxin β receptor activation promotes mRNA expression of RelA and pro-inflammatory cytokines TNFα and IL-1β in bladder cancer cells.

Islet α-cell Inflammation Induced By NF-κB inducing kinase (NIK) Leads to Hypoglycemia, Pancreatitis, Growth Retardation, and Postnatal Death in Mice.

A LIGHT-HVEM/LTβR axis contributes to the fibrosis of intrauterine adhesion