Validation Data Gallery
Tested Applications
| Positive WB detected in | human saliva, HEK-293 cells, human pancreas tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human liver tissue, human prostate hyperplasia tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 7 publications below |
| IHC | See 4 publications below |
| IF | See 1 publications below |
Product Information
10933-1-AP targets Lactotransferrin/LTF in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Lactotransferrin/LTF fusion protein Ag1379 相同性解析による交差性が予測される生物種 |
| Full Name | lactotransferrin |
| Calculated molecular weight | 80 kDa |
| Observed molecular weight | 30,51,80 kDa |
| GenBank accession number | BC015822 |
| Gene Symbol | LTF |
| Gene ID (NCBI) | 4057 |
| RRID | AB_2139227 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P02788 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
LTF(Lactotransferrin) is also named as GIG12, LF and belongs to the transferrin family. It is an iron binding transport protein which can bind two atoms of ferric iron in association with the binding of an anion, usually bicarbonate. LTF is also an important protein component of the innate immune system that is broadly distributed within the body fluids. It can contribute to the activation of both the innate and adaptive immune responses by promoting the recruitment of leukocytes and activation of dendritic cells(PMID:18453607). This protein has 2 isoforms produced by alternative promoter usage. The human LTF can be isolated two fragments, an N-terminal tryptic fragment having an MW of 30 kDa and a C-terminal tryptic fragment having an MW of 50 kDa by a mild tryptic digest(PMID:3790094).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Lactotransferrin/LTF antibody 10933-1-AP | Download protocol |
| IHC protocol for Lactotransferrin/LTF antibody 10933-1-AP | Download protocol |
| IP protocol for Lactotransferrin/LTF antibody 10933-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Sci Adv Mucus concentration-dependent biophysical abnormalities unify submucosal gland and superficial airway dysfunction in cystic fibrosis. | ||
Cancer Cell Int Identification and validation of an immune-related prognostic signature and key gene in papillary thyroid carcinoma. | ||
Biochem Biophys Res Commun NEDD4L-mediated LTF protein degradation limits ferroptosis.
| ||
Int Med Case Rep J Collagen fiber changes related to keratoconus with secondary corneal amyloidosis. | ||
Bioact Mater Passively-targeted mitochondrial tungsten-based nanodots for efficient acute kidney injury treatment |