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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LUC7L Recombinant antibody (83585-1-RR)

    WB analysis using 83585-1-RR (same clone as 83585-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83585-1-RR (LUC7L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83585-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using LUC7L Recombinant antibody (83585-1-RR)

    WB analysis of HepG2 using 83585-1-RR (same clone as 83585-1-PBS)

    WB result of LUC7L antibody (83585-1-RR; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LUC7L transfected HepG2 cells. This data was developed using the same antibody clone with 83585-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using LUC7L Recombinant antibody (83585-1-RR)

    IHC staining of rat brain using 83585-1-RR (same clone as 83585-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 83585-1-RR (LUC7L antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83585-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using LUC7L Recombinant antibody (83585-1-RR)

    IHC staining of rat brain using 83585-1-RR (same clone as 83585-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 83585-1-RR (LUC7L antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83585-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using LUC7L Recombinant antibody (83585-1-RR)

    IF Staining of HepG2 using 83585-1-RR (same clone as 83585-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using LUC7L antibody (83585-1-RR, Clone: 240311B10 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83585-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of MCF-7 cells using LUC7L Recombinant antibody (83585-1-RR)

    FC experiment of MCF-7 using 83585-1-RR (same clone as 83585-1-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug LUC7L Recombinant antibody (83585-1-RR, Clone:240311B10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83585-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83585-1-RR

    Affinity and Kinetic Characterization of 83585-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83585-1-RR against Human LUC7L were performed. The affinity constant is 99.9 pM.

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  • Featured Product
  • KD/KO Validated

LUC7L Recombinant antibody, PBS Only

LUC7L Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 83585-1-PBS
Clone No.240311B10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

Putative RNA-binding protein Luc7-like 1, LUC7L1, LUC7B1, LUC7 LIKE, hLuc7B1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83585-1-PBS targets LUC7L in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen LUC7L fusion protein Ag9170 相同性解析による交差性が予測される生物種
Full Name LUC7-like (S. cerevisiae)
Calculated molecular weight 44 kDa
Observed molecular weight38 kDa and 44 kDa
GenBank accession numberBC065198
Gene Symbol LUC7L
Gene ID (NCBI) 55692
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9NQ29
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

LUC7L, also known as LUC7L1, belongs to the Luc7 family. LUC7L encodes a putative RNA binding protein similar to the yeast Luc7p subunit of the U1 snRNP splicing complex, which is normally required for 5` splice site selection (PMID: 15474286). LUC7L is widely expressed. LUC7L has 3 isoforms with the molecular mass of 38, 42 and 44 kDa.