LXN Polyclonal antibody
LXN Polyclonal Antibody for WB, IHC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF, IP, ELISA
Conjugate
Unconjugated
Cat no : 13056-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse lung tissue |
| Positive IP detected in | mouse lung tissue |
| Positive IHC detected in | human intrahepatic cholangiocarcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IF | See 2 publications below |
Product Information
13056-1-AP targets LXN in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | LXN fusion protein Ag3726 相同性解析による交差性が予測される生物種 |
| Full Name | latexin |
| Calculated molecular weight | 222 aa, 26 kDa |
| Observed molecular weight | 26 kDa |
| GenBank accession number | BC005346 |
| Gene symbol | LXN |
| Gene ID (NCBI) | 56925 |
| RRID | AB_2139511 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Latexin, also named as LTXN, LXN, ECI, MUM and TCI, is hardly reversible, non-competitive, and potent inhibitor of CPA1, CPA2 and CPA4. It may function as a neuroprotective mechanism against tau toxicity.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for LXN antibody 13056-1-AP | Download protocol |
| IHC protocol for LXN antibody 13056-1-AP | Download protocol |
| IP protocol for LXN antibody 13056-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Brain Res Astrocytic neuroprotection through induction of cytoprotective molecules; a proteomic analysis of mutant P301S tau-transgenic mouse. | ||
Cell Rep Latexin regulates sex dimorphism in hematopoiesis via gender-specific differential expression of microRNA 98-3p and thrombospondin 1 | ||
Nat Neurosci Derivation and transcriptional reprogramming of border-forming wound repair astrocytes after spinal cord injury or stroke in mice |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Kyosuke (Verified Customer) (06-12-2019) | It works for IP study.
|
FH Kyosuke (Verified Customer) (06-05-2019) | It works for WB and IP very well.
|
