LXN Monoclonal antibody

pig brain tissue were subjected to SDS PAGE followed by western blot with 66957-1-Ig (LXN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

HeLa cells were subjected to SDS PAGE followed by western blot with 66957-1-Ig (LXN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

A549 cells were subjected to SDS PAGE followed by western blot with 66957-1-Ig (LXN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

HEK-293 cells were subjected to SDS PAGE followed by western blot with 66957-1-Ig (LXN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 66957-1-Ig (LXN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

rabbit brain tissue were subjected to SDS PAGE followed by western blot with 66957-1-Ig (LXN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66957-1-Ig (LXN antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66957-1-Ig (LXN antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using LXN antibody (66957-1-Ig, Clone: 3E5A5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human LXN (66957-1-Ig, Clone:3E5A5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).