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  • KD/KO Validated

LY6E Polyclonal antibody

LY6E Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 22144-1-AP

Synonyms

RIG-E, RIGE, Retinoic acid-induced gene E protein, Ly-6E, Ly 6E


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Tested Applications

Positive WB detected inmouse kidney tissue, rat kidney tissue
Positive IHC detected inhuman tonsillitis tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22144-1-AP targets LY6E in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LY6E fusion protein Ag17573 相同性解析による交差性が予測される生物種
Full Name lymphocyte antigen 6 complex, locus E
Calculated molecular weight 131 aa, 14 kDa
Observed molecular weight12-16 kDa
GenBank accession numberBC119708
Gene symbol LY6E
Gene ID (NCBI) 4061
RRIDAB_2879007
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The predicted MW of LY6E is 13 kDa. Catalog#22144-1-AP recognises two band of 13 kDa and 18 kDa, and the additional 18 kDa band may be due to glycosylation.

Protocols

Product Specific Protocols
WB protocol for LY6E antibody 22144-1-APDownload protocol
IHC protocol for LY6E antibody 22144-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Lab Chip

LY6E protein facilitates adeno-associated virus crossing in a biomimetic chip model of the human blood-brain barrier

Authors - Dan Liu
humanWB,IHC

Carcinogenesis

α5-nAChR associated with Ly6E modulates cell migration via TGF-β1/Smad signaling in non-small cell lung cancer.

Authors - Qian Zhang
humanWB

J Virol

LY6E Restricts the Entry of Human Coronaviruses, Including the Currently Pandemic SARS-CoV-2.

Authors - Xuesen Zhao
  • KD Validated
humanWB

J Virol

Characterization of CCoV-HuPn-2018 spike protein-mediated viral entry

Authors - Yongmei Liu