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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

LY6G6F Polyclonal antibody

LY6G6F Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 25872-1-AP

Synonyms

C6orf21, G6f, LY6G6D, LY6G6F, NG32

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using LY6G6F Polyclonal antibody (25872-1-AP)

    WB analysis of HepG2 using 25872-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 25872-1-AP (LY6G6F Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HL-60 cells using LY6G6F Polyclonal antibody (25872-1-AP)

    WB analysis of HL-60 using 25872-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 25872-1-AP (LY6G6F Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inHepG2 cells, HL-60 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

The immunogen of 25872-1-AP is LY6G6F Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LY6G6F fusion protein Ag22467 相同性解析による交差性が予測される生物種
Full Name lymphocyte antigen 6 complex, locus G6F
Calculated molecular weight 297 aa, 32 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC137212
Gene symbol LY6G6F
Gene ID (NCBI) 259215
RRIDAB_2880278
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LY6G6F, also named as C6orf21, G6F, LY6G6D and NG32, plays a role in the downstream signal transduction pathways involving GRB2 and GRB7. It has monomer (~30 kda) and homodimer (~60 kDa).

Protocols

Product Specific Protocols
WB protocol for LY6G6F antibody 25872-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Arterioscler Thromb Vasc Biol

Caspase-8 Promotes Pulmonary Hypertension by Activating Macrophage-Associated Inflammation and IL-1β (Interleukin 1β) Production.

Authors - Wuwei Rong
View Article