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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

LY6H Polyclonal antibody

LY6H Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 23933-1-AP

Synonyms

Ly 6H, LY6H, Lymphocyte antigen 6H

Validation Data Gallery

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  • Western Blot (WB) analysis of Raji cells using LY6H Polyclonal antibody (23933-1-AP)

    WB analysis of Raji using 23933-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 23933-1-AP (LY6H Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using LY6H Polyclonal antibody (23933-1-AP)

    IHC staining of human testis using 23933-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis slide using 23933-1-AP (LY6H Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using LY6H Polyclonal antibody (23933-1-AP)

    IHC staining of human testis using 23933-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis slide using 23933-1-AP (LY6H Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using LY6H Polyclonal antibody (23933-1-AP)

    IHC staining of mouse brain using 23933-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 23933-1-AP (LY6H antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using LY6H Polyclonal antibody (23933-1-AP)

    IHC staining of mouse brain using 23933-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 23933-1-AP (LY6H antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inRaji cells
Positive IHC detected inhuman testis tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

23933-1-AP targets LY6H in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LY6H fusion protein Ag20762 相同性解析による交差性が予測される生物種
Full Name lymphocyte antigen 6 complex, locus H
Calculated molecular weight 15 kDa
Observed molecular weight 16 kDa
GenBank accession numberBC028894
Gene symbol LY6H
Gene ID (NCBI) 4062
RRIDAB_2879366
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LY6H is highly expressed in particular subdivisions of human brain and also in MOLT-3 and -4 acute lymphoblastic leukemia cells. It has some isoforms with MW 14-16 kDa.

Protocols

Product Specific Protocols
WB protocol for LY6H antibody 23933-1-APDownload protocol
IHC protocol for LY6H antibody 23933-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols