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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

LY6K Polyclonal antibody

LY6K Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 12026-1-AP

Synonyms

Lymphocyte antigen 6K, Ly-6K, Ly 6K, HSJ001348, CO16

Validation Data Gallery

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  • Western Blot (WB) analysis of human testis tissue using LY6K Polyclonal antibody (12026-1-AP)

    WB analysis of human testis using 12026-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 12026-1-AP (LY6K antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using LY6K Polyclonal antibody (12026-1-AP)

    WB analysis of human testis using 12026-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 12026-1-AP (LY6K antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of PC-3 cells using LY6K Polyclonal antibody (12026-1-AP)

    IP experiment of PC-3 using 12026-1-AP

    IP result of anti-LY6K (IP:12026-1-AP, 4ug; Detection:12026-1-AP 1:300) with PC-3 cells lysate 3200ug.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using LY6K Polyclonal antibody (12026-1-AP)

    IHC staining of human skin cancer using 12026-1-AP

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 12026-1-AP (LY6K antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inhuman testis tissue
Positive IP detected inPC-3 cells
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

12026-1-AP targets LY6K in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivitybovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LY6K fusion protein Ag2656 相同性解析による交差性が予測される生物種
Full Name lymphocyte antigen 6 complex, locus K
Calculated molecular weight 179 aa, 20 kDa
Observed molecular weight 28 kDa
GenBank accession numberBC001291
Gene symbol LY6K
Gene ID (NCBI) 54742
RRIDAB_2877817
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for LY6K antibody 12026-1-APDownload protocol
IHC protocol for LY6K antibody 12026-1-APDownload protocol
IP protocol for LY6K antibody 12026-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
bovineWB

Int J Biol Macromol

TMT-based quantitative N-glycoproteomic analysis reveals glycoprotein protection can improve the quality of frozen bovine sperm.

Authors - Na Wang
View Article