LY96/MD2 Polyclonal antibody
LY96/MD2 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF, IP, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 11784-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse testis tissue, rat testis tissue |
Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 7 publications below |
IHC | See 2 publications below |
IF | See 2 publications below |
IP | See 1 publications below |
CoIP | See 2 publications below |
Product Information
11784-1-AP targets LY96/MD2 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | LY96/MD2 fusion protein Ag2447 相同性解析による交差性が予測される生物種 |
Full Name | lymphocyte antigen 96 |
Calculated molecular weight | 160 aa, 18 kDa |
Observed molecular weight | 20-26 kDa |
GenBank accession number | BC020690 |
Gene symbol | MD2 |
Gene ID (NCBI) | 23643 |
RRID | AB_2138089 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
LY96, also named as ESOP1 or MD 2, is a small secreted glycoprotein that can bind to both the hydrophobic portion of LPS and to the extracellular domain of TLR4. The interaction between MD-2 and LPS bridges the two TLR4 molecules and induces the dimerization of LPS-MD-2-TLR4, which forms the structural basis for biological functions of TLR4/MD-2 complex. Due to its essential role in mediating the interaction between LPS and TLR4, MD-2 has been extensively explored as a therapeutic target for treatment of inflammatory disorders.
Protocols
Product Specific Protocols | |
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WB protocol for LY96/MD2 antibody 11784-1-AP | Download protocol |
IHC protocol for LY96/MD2 antibody 11784-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Front Oncol MD2 Is a Potential Biomarker Associated with Immune Cell Infiltration in Gliomas. | ||
J Cardiovasc Transl Res Macrophage CD36 and TLR4 Cooperation Promotes Foam Cell Formation and VSMC Migration and Proliferation Under Circadian Oscillations. | ||
Molecules Sal B Alleviates Myocardial Ischemic Injury by Inhibiting TLR4 and the Priming Phase of NLRP3 Inflammasome. | ||
Oncol Rep Curcumin inhibits cell proliferation and migration in NSCLC through a synergistic effect on the TLR4/MyD88 and EGFR pathways. | ||
J Agric Food Chem Biotransformation of 18β-Glycyrrhetinic Acid by Human Intestinal Fungus Aspergillus niger RG13B1 and the Potential Anti-Inflammatory Mechanism of Its Metabolites | ||
Bioorg Chem The antimicrobial peptide LK2(6)A(L) exhibits anti-inflammatory activity by binding to the myeloid differentiation 2 domain and protects against LPS-induced acute lung injury in mice |