Validation Data Gallery

Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis of HeLa using 18135-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 18135-1-AP (LYN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HL-60 using 18135-1-AP
HL-60 cells were subjected to SDS PAGE followed by western blot with 18135-1-AP (LYN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 18135-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 18135-1-AP (LYN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 18135-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 18135-1-AP (LYN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of L02 using 18135-1-AP
L02 cells were subjected to SDS PAGE followed by western blot with 18135-1-AP (LYN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 18135-1-AP
IP result of anti-LYN (IP:18135-1-AP, 4ug; Detection:18135-1-AP 1:1000) with HeLa cells lysate 1200ug.
IHC staining of human kidney using 18135-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18135-1-AP (LYN Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human kidney using 18135-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18135-1-AP (LYN Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human hepatocirrhosis using 18135-1-AP
Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 18135-1-AP (LYN Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human hepatocirrhosis using 18135-1-AP
Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 18135-1-AP (LYN Antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HeLa using 18135-1-AP
Immunofluorescent analysis of HeLa cells using 18135-1-AP (LYN antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of HeLa using 18135-1-AP
1x10^6 HeLa cells were intracellularly stained with 0.25 ug LYN Polyclonal antibody (18135-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

LYN Polyclonal antibody

LYN Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 18135-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Synonyms

V-yes-1 Yamaguchi sarcoma viral related oncogene homolog, Tyrosine-protein kinase Lyn, Tyrosine protein kinase Lyn, Lck/Yes-related novel protein tyrosine kinase, JTK8

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (2)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, HL-60 cells, Jurkat cells, K-562 cells, L02 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human kidney tissue, human hepatocirrhosis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells
Positive FC (Intra) detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 1 publications below
IHC	See 1 publications below
IF	See 1 publications below

Product Information

18135-1-AP targets LYN in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	LYN fusion protein Ag12723 相同性解析による交差性が予測される生物種
Full Name	v-yes-1 Yamaguchi sarcoma viral related oncogene homolog
Calculated molecular weight	512 aa, 59 kDa
Observed molecular weight	53-56 kDa, 66-70 kDa
GenBank accession number	BC075002
Gene Symbol	LYN
Gene ID (NCBI)	4067
RRID	AB_11042319
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P07948
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

LYN, also named as JTK8, p53Lyn and p56Lyn, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. It is a non-receptor tyrosine-protein kinase that transmits signals from cell surface receptors and plays an important role in the regulation of innate and adaptive immune responses, hematopoiesis, responses to growth factors and cytokines, integrin signaling, but also responses to DNA damage and genotoxic agents. LYN is a component of the mTOR (mammalian target of rapamycin) pathway.

Protocols

Product Specific Protocols
WB protocol for LYN antibody 18135-1-AP	Download protocol
IHC protocol for LYN antibody 18135-1-AP	Download protocol
IF protocol for LYN antibody 18135-1-AP	Download protocol
IP protocol for LYN antibody 18135-1-AP	Download protocol
FC protocol for LYN antibody 18135-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IF	J Invest Dermatol Loss of LYN is frequent in targeted-therapy resistant melanoma cells and favors metastatic properties Authors - Céline Gaudel KD Validated View Article
human	IHC	Front Cell Dev Biol Immune Characteristics of LYN in Tumor Microenvironment of Gliomas. Authors - Chonghua Jiang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HeLa using 18135-1-AP

WB analysis of HL-60 using 18135-1-AP

WB analysis of Jurkat using 18135-1-AP

WB analysis of K-562 using 18135-1-AP

WB analysis of L02 using 18135-1-AP

IP experiment of HeLa using 18135-1-AP

IHC staining of human kidney using 18135-1-AP