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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LYPD1 Polyclonal antibody (11415-1-AP)

    WB analysis using 11415-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11415-1-AP (LYPD1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using LYPD1 Polyclonal antibody (11415-1-AP)

    IHC staining of human ovary cancer using 11415-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 11415-1-AP (LYPD1 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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LYPD1 Polyclonal antibody

LYPD1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 11415-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, ELISA

Synonyms

PSEC0181, PHTS, LYPDC1, Lynx2, Ly6/PLAUR domain-containing protein 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11415-1-AP targets LYPD1 in WB, IHC, ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LYPD1 fusion protein Ag1965 相同性解析による交差性が予測される生物種
Full Name LY6/PLAUR domain containing 1
Calculated molecular weight 141 aa, 15 kDa
Observed molecular weight15 kDa
GenBank accession numberBC017318
Gene Symbol LYPD1
Gene ID (NCBI) 116372
RRIDAB_3085372
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N2G4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LY6/PLAUR domain containing 1 (LYPD1) is a highly conserved glycosylphosphatidylinositol (GPI)-anchored protein. LYPD1 contains one cysteine-rich extracellular leukocyte antigen-6 (Ly6)/uPAR (PLAUR) domain followed by signal sequence that triggers posttranslational GPI-anchor addition incorporating mature, LYPD1 into the plasma membrane. LYPD1 is believed to act as a modulator of nicotinic acetylcholine receptors (nAChRs) activity. LYPD1 increases receptor desensitization in vitro and decreases affinity for ACh of alpha-4:beta-2-containing nAChRs. LYPD1 has 4 isoforms produced by alternative splicing (PMID: 33536191).

Protocols

Product Specific Protocols
WB protocol for LYPD1 antibody 11415-1-APDownload protocol
IHC protocol for LYPD1 antibody 11415-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols