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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

LYVE1 Polyclonal antibody, PBS Only

LYVE1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 28321-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LYVE1 Polyclonal antibody (28321-1-AP)

    WB analysis using 28321-1-AP (same clone as 28321-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28321-1-AP (LYVE1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28321-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using LYVE1 Polyclonal antibody (28321-1-AP)

    WB analysis using 28321-1-AP (same clone as 28321-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28321-1-AP (LYVE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28321-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat liver tissue using LYVE1 Polyclonal antibody (28321-1-AP)

    IHC staining of rat liver using 28321-1-AP (same clone as 28321-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 28321-1-AP (LYVE1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28321-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat liver tissue using LYVE1 Polyclonal antibody (28321-1-AP)

    IHC staining of rat liver using 28321-1-AP (same clone as 28321-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 28321-1-AP (LYVE1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28321-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

28321-1-PBS targets LYVE1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LYVE1 fusion protein Ag28131 相同性解析による交差性が予測される生物種
Full Name lymphatic vessel endothelial hyaluronan receptor 1
Calculated molecular weight 322 aa, 35 kDa
Observed molecular weight70 kDa
GenBank accession numberBC026231
Gene symbol LYVE1
Gene ID (NCBI) 10894
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

LYVE1 is a 322-amino acid type-I integral membrane glycoprotein primarily expressed on lymphatic endothelial cells. LYVE1 is a CD44 homologue. It acts as a receptor and binds to both soluble and immobilized hyaluronan. LYVE1 may function in lymphatic hyaluronan transport and have a role in tumor metastasis.