Lysozyme Polyclonal antibody

Lysozyme Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 15013-1-AP

Synonyms

LYZ, Muramidase, LZM, Lysozyme C, EC:3.2.1.17


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Tested Applications

Positive WB detected inTHP-1 cells, mouse lung tissue, human spleen tissue, HL-60 cells
Positive IHC detected inmouse heart tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15013-1-AP targets Lysozyme in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Lysozyme fusion protein Ag6917 相同性解析による交差性が予測される生物種
Full Name lysozyme (renal amyloidosis)
Calculated molecular weight 17 kDa
Observed molecular weight15-17 kDa
GenBank accession numberBC004147
Gene symbol Lysozyme
Gene ID (NCBI) 4069
RRIDAB_10639507
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LYZ(Lysozyme C) is also named as LZM and belongs to the glycosyl hydrolase 22 family. It catalyzes the hydrolysis of the bacterial cell wall beta(1-4) glycosidic linkages between N-acetylmuramic acid and N-acetylglucosamine. In tissues and body fluids this protein is associated with the monocyte-macrophage system and enhance the activity of immunoagents. LYZ is capable of both hydrolysis and transglycosylation; it shows also a slight esterase activity. Defects in LYZ are a cause of amyloidosis type 8 (AMYL8). The full length protein has a signal peptide with 18 amino acid.

Protocols

Product Specific Protocols
WB protocol for Lysozyme antibody 15013-1-APDownload protocol
IHC protocol for Lysozyme antibody 15013-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Cell Chem Biol

Engineered Glycocalyx Regulates Stem Cell Proliferation in Murine Crypt Organoids.

Authors - Sara H Rouhanifard
human

Arch Toxicol

Extending the viability of human precision-cut intestinal slice model for drug metabolism studies.

Authors - C Biel
mouseWB

FASEB J

Loss of interleukin-10 receptor disrupts intestinal epithelial cell proliferation and skews differentiation towards the goblet cell fate.

Authors - Brittany R Jenkins
mouseWB

Arch Med Sci

Lysozyme promotes renal fibrosis through the JAK/STAT3 signal pathway in diabetic nephropathy

Authors - Yan Ren
mouse

Int J Mol Sci

Nrf2-Knockout Protects from Intestinal Injuries in C57BL/6J Mice Following Abdominal Irradiation with γ Rays.

Authors - Wenyan Yang
ratIF

Physiol Genomics

The Atg16l1 gene: characterization of wild type, knock-in, and knock-out phenotypes in rats.

Authors - Kari Chesney

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Balawant (Verified Customer) (12-20-2022)

I have used this antibody for colon tissue and colon cell line. this antibody is working great and no background.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: colon tissue and colon cell line (caco2 cells)
FH

Claudio (Verified Customer) (12-15-2021)

The antibody staining did not yield any labelling, though it was tested in paraffin sections so you might get better results with cryostat sections.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Mouse heart sections