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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Lamin B2 Monoclonal antibody, PBS Only

Lamin B2 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

rat, mouse, human

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2C7B1

Cat no : 68475-1-PBS

Synonyms

LMNB2, LMN2, Lamin-B2, LAMB2, 2C7B1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Lamin B2 Monoclonal antibody (68475-1-Ig)

    WB analysis using 68475-1-Ig (same clone as 68475-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68475-1-Ig (Lamin B2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68475-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Lamin B2 Monoclonal antibody (68475-1-Ig)

    IF Staining of HepG2 using 68475-1-Ig (same clone as 68475-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Lamin B2 antibody (68475-1-Ig, Clone: 2C7B1 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 68475-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68475-1-PBS targets Lamin B2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with rat, mouse, human samples.

Tested Reactivity rat, mouse, human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Lamin B2 fusion protein Ag33296 相同性解析による交差性が予測される生物種
Full Name lamin B2
Calculated molecular weight 68 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC006551
Gene symbol Lamin B2
Gene ID (NCBI) 84823
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Lamins are nuclear membrane structural components that are important in structural integrity of the nucleus and may also interact with chromatin (PMID: 33033404). Research studies show that lamin B2 knockout mice exhibit neuronal developmental defects and that both proteins are essential for typical brain development (PMID: 20145110). Mutations in Lamin B2 can result in a susceptibility to developing acquired partial lipodystrophy, a rare disorder characterized by the progressive loss of subcutaneous fat in a bilaterally symmetrical fashion (PMID: 16826530).