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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Lamin B2 Recombinant antibody

Lamin B2 Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230184E7

Cat no : 82801-2-RR

Synonyms

LMNB2, LMN2, Lamin-B2, LAMB2, 230184E7

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Lamin B2 Recombinant antibody (82801-2-RR)

    WB analysis using 82801-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82801-2-RR (Lamin B2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using Lamin B2 Recombinant antibody (82801-2-RR)

    IHC staining of human stomach cancer using 82801-2-RR

    Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 82801-2-RR (Lamin B2 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Lamin B2 Recombinant antibody (82801-2-RR)

    IF Staining of HepG2 using 82801-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Lamin B2 antibody (82801-2-RR, Clone: 230184E7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of HepG2 cells using Lamin B2 Recombinant antibody (82801-2-RR)

    FC experiment of HepG2 using 82801-2-RR

    (blue)1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Anti-Human Lamin B2 (82801-2-RR, Clone:230184E7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:166(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 82801-2-RR

    Affinity and Kinetic Characterization of 82801-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82801-2-RR against Human Lamin B2 were performed. The affinity constant is 2.81 nM.

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Tested Applications

Positive WB detected inHeLa cells, COLO 320 cells, HL-60 cells, Jurkat cells, HT-1080 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82801-2-RR targets Lamin B2 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Lamin B2 fusion protein Ag1335 相同性解析による交差性が予測される生物種
Full Name lamin B2
Calculated molecular weight 68 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC006551
Gene symbol Lamin B2
Gene ID (NCBI) 84823
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Lamins are nuclear membrane structural components that are important in structural integrity of the nucleus and may also interact with chromatin (PMID: 33033404). Research studies show that lamin B2 knockout mice exhibit neuronal developmental defects and that both proteins are essential for typical brain development (PMID: 20145110). Mutations in Lamin B2 can result in a susceptibility to developing acquired partial lipodystrophy, a rare disorder characterized by the progressive loss of subcutaneous fat in a bilaterally symmetrical fashion (PMID: 16826530).

Protocols

Product Specific Protocols
WB protocol for Lamin B2 antibody 82801-2-RRDownload protocol
IHC protocol for Lamin B2 antibody 82801-2-RRDownload protocol
IF protocol for Lamin B2 antibody 82801-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols