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Lin28B-specific Polyclonal antibody
Lin28B-specific Polyclonal Antibody for IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (2)
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 16178-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive IHC detected in | human colon cancer tissue, human brain tissue, human spleen tissue, human testis tissue, human kidney tissue, human lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 11 publications below |
| IHC | See 12 publications below |
| IF | See 3 publications below |
Product Information
16178-1-AP targets Lin28B-specific in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | lin-28 homolog B (C. elegans) |
| Calculated molecular weight | 28 kDa |
| Observed molecular weight | 27 kDa |
| GenBank accession number | NM_001004317 |
| Gene symbol | LIN28B |
| Gene ID (NCBI) | 389421 |
| RRID | AB_2135051 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
LIN28B, also named as CSDD2, belongs to the lin-28 family. It acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7). LIN2B is specifically expressed in embryonic muscle, neurons, and epithelia, as well as in embryonic carcinoma cells. However, it is suppressed in most adult tissues except for skeletal and cardiac muscle. LIN2B shares 78% sequence identity with LIN28A. This antibody is specific to LIN28B.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Lin28B-specific antibody 16178-1-AP | Download protocol |
| IF protocol for Lin28B-specific antibody 16178-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancer Res Activation of the Lin28/let-7 Axis by Loss of ESE3/EHF Promotes a Tumorigenic and Stem-like Phenotype in Prostate Cancer. | ||
EMBO Rep Lin28 enhances de novo fatty acid synthesis to promote cancer progression via SREBP-1.
| ||
Gastric Cancer LOC101929709 promotes gastric cancer progression by aiding LIN28B to stabilize c-MYC mRNA | ||
Sci Rep Testicular expression of the Lin28/let-7 system: Hormonal regulation and changes during postnatal maturation and after manipulations of puberty. | ||
Am J Cancer Res Cellular 5-hydroxylmethylcytosine content determines tumorigenic potential and prognosis of pancreatic ductal adenocarcinoma. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Sarah (Verified Customer) (01-24-2023) | Antibody worked well
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