Validation Data Gallery
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Tested Applications
| Positive IHC detected in | human colon cancer tissue, human kidney tissue, human lung tissue, human spleen tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 12 publications below |
| IHC | See 12 publications below |
| IF | See 3 publications below |
| RIP | See 1 publications below |
Product Information
16178-1-AP targets Lin28B-specific in WB, IHC, IF/ICC, RIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | lin-28 homolog B (C. elegans) |
| Calculated molecular weight | 28 kDa |
| GenBank accession number | NM_001004317 |
| Gene Symbol | LIN28B |
| Gene ID (NCBI) | 389421 |
| RRID | AB_2135051 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6ZN17 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
LIN28B, also named as CSDD2, belongs to the lin-28 family. It acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7). LIN2B is specifically expressed in embryonic muscle, neurons, and epithelia, as well as in embryonic carcinoma cells. However, it is suppressed in most adult tissues except for skeletal and cardiac muscle. LIN2B shares 78% sequence identity with LIN28A. This antibody is specific to LIN28B.u
Publications
| Species | Application | Title |
|---|---|---|
Cancer Res Activation of the Lin28/let-7 Axis by Loss of ESE3/EHF Promotes a Tumorigenic and Stem-like Phenotype in Prostate Cancer. | ||
Exp Mol Med RNA m5C modification upregulates E2F1 expression in a manner dependent on YBX1 phase separation and promotes tumor progression in ovarian cancer | ||
J Clin Invest Liver cancer initiation requires translational activation by an oncofetal regulon involving LIN28 proteins | ||
Cancer Lett LIN28B enhances the chemosensitivity of colon cancer cells via inducing genomic instability by upsetting the balance between the production and removal of reactive oxygen species | ||