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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NGAL Polyclonal antibody

NGAL Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 31721-1-AP

Synonyms

lipocalin 2, LCN2, Lcn 2, HNL, 25 kDa alpha-2-microglobulin-related subunit of MMP-9

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Lipocalin-2/NGAL Polyclonal antibody (31721-1-AP)

    WB analysis using 31721-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31721-1-AP (Lipocalin-2/NGAL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inHT-29 cells, mouse bone marrow tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31721-1-AP targets NGAL in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name lipocalin 2
Calculated molecular weight23kDa
Observed molecular weight22 kDa
GenBank accession numberNM_005564.5
Gene symbol NGAL/LCN2
Gene ID (NCBI) 3934
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Lipocalin-2 (LCN-2) is a adipocytokine also referred to as neutrophil gelatinase-associated lipocalin (NGAL). Lipocalin-2 is a circulatory protein responsible for the transportation of small and hydrophobic molecules to target organs. Lipocalin-2 is used as a biomarker for acute and chronic renal injury. It is present in a large variety of cells including neutrophil, hepatocytes, lung, bone marrow, adipose tissue, macrophages, thymus, non-neoplastic breast duct, prostate, and renal cells. Different functions have been associated with Lipocalin-2, including antibacterial, anti-inflammatory, and protection against cell and tissue stress (PMID:34463264).

Protocols

Product Specific Protocols
WB protocol for NGAL antibody 31721-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols