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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Lysozyme Polyclonal antibody, PBS Only

Lysozyme Polyclonal Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

Cat no : 15013-1-PBS

Synonyms

LYZ, Muramidase, LZM, Lysozyme C, EC:3.2.1.17

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse lung tissue using Lysozyme Polyclonal antibody (15013-1-AP)

    WB analysis of mouse lung using 15013-1-AP (same clone as 15013-1-PBS)

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 15013-1-AP (LYZ antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15013-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human spleen tissue using Lysozyme Polyclonal antibody (15013-1-AP)

    WB analysis of human spleen using 15013-1-AP (same clone as 15013-1-PBS)

    human spleen tissue were subjected to SDS PAGE followed by western blot with 15013-1-AP (LYZ antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15013-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HL-60 cells using Lysozyme Polyclonal antibody (15013-1-AP)

    WB analysis of HL-60 using 15013-1-AP (same clone as 15013-1-PBS)

    HL-60 cells were subjected to SDS PAGE followed by western blot with 15013-1-AP (LYZ antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15013-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of THP-1 cells using Lysozyme Polyclonal antibody (15013-1-AP)

    WB analysis of THP-1 using 15013-1-AP (same clone as 15013-1-PBS)

    THP-1 cells were subjected to SDS PAGE followed by western blot with 15013-1-AP (Lysozyme antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15013-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using Lysozyme Polyclonal antibody (15013-1-AP)

    IHC staining of mouse heart using 15013-1-AP (same clone as 15013-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15013-1-AP (Lysozyme antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15013-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using Lysozyme Polyclonal antibody (15013-1-AP)

    IHC staining of mouse heart using 15013-1-AP (same clone as 15013-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15013-1-AP (Lysozyme antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15013-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using Lysozyme Polyclonal antibody (15013-1-AP)

    IHC staining of human stomach cancer using 15013-1-AP (same clone as 15013-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15013-1-AP (Lysozyme antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15013-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15013-1-PBS targets Lysozyme in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Lysozyme fusion protein Ag6917 相同性解析による交差性が予測される生物種
Full Name lysozyme (renal amyloidosis)
Calculated molecular weight 17 kDa
Observed molecular weight15-17 kDa
GenBank accession numberBC004147
Gene symbol Lysozyme
Gene ID (NCBI) 4069
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

LYZ(Lysozyme C) is also named as LZM and belongs to the glycosyl hydrolase 22 family. It catalyzes the hydrolysis of the bacterial cell wall beta(1-4) glycosidic linkages between N-acetylmuramic acid and N-acetylglucosamine. In tissues and body fluids this protein is associated with the monocyte-macrophage system and enhance the activity of immunoagents. LYZ is capable of both hydrolysis and transglycosylation; it shows also a slight esterase activity. Defects in LYZ are a cause of amyloidosis type 8 (AMYL8). The full length protein has a signal peptide with 18 amino acid.