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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Lysozyme Recombinant antibody

Lysozyme Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241054E1

Cat no : 84140-1-RR

Synonyms

LYZ, LZM, Lysozyme C, EC:3.2.1.17, 241054E1

Validation Data Gallery

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  • Western Blot (WB) analysis of human saliva using Lysozyme Recombinant antibody (84140-1-RR)

    WB analysis of human saliva using 84140-1-RR

    human saliva were subjected to SDS PAGE followed by western blot with 84140-1-RR (Lysozyme antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84140-1-RR

    Affinity and Kinetic Characterization of 84140-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84140-1-RR against Human Lysozyme were performed. The affinity constant is 133 nM.

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Tested Applications

Positive WB detected inhuman saliva

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84140-1-RR is Lysozyme Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Lysozyme fusion protein Ag6917 相同性解析による交差性が予測される生物種
Full Name lysozyme (renal amyloidosis)
Calculated molecular weight 17 kDa
Observed molecular weight10 kDa
GenBank accession numberBC004147
Gene symbol Lysozyme
Gene ID (NCBI) 4069
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LYZ(Lysozyme C) is also named as LZM and belongs to the glycosyl hydrolase 22 family. It catalyzes the hydrolysis of the bacterial cell wall beta(1-4) glycosidic linkages between N-acetylmuramic acid and N-acetylglucosamine. In tissues and body fluids this protein is associated with the monocyte-macrophage system and enhance the activity of immunoagents. LYZ is capable of both hydrolysis and transglycosylation; it shows also a slight esterase activity. Defects in LYZ are a cause of amyloidosis type 8 (AMYL8). The full length protein has a signal peptide with 18 amino acid.

Protocols

Product Specific Protocols
WB protocol for Lysozyme antibody 84140-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols