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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using M-cadherin Polyclonal antibody (29928-1-AP)

    WB analysis using 29928-1-AP (same clone as 29928-1-PBS)

    C2C12 cells were subjected to SDS PAGE followed by western blot with 29928-1-AP (M-cadherin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29928-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse skeletal muscle tissue using M-cadherin Polyclonal antibody (29928-1-AP)

    IF Staining of mouse skeletal muscle using 29928-1-AP (same clone as 29928-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using M-cadherin antibody (29928-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29928-1-PBS in a different storage buffer formulation.

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M-cadherin Polyclonal antibody, PBS Only

M-cadherin Polyclonal Antibody for WB, IF-P, Indirect ELISA
Cat No. 29928-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF-P, Indirect ELISA

Synonyms

CDH15, Cadherin 14, Cadherin 15, Cadherin-14, Cadherin-15

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

29928-1-PBS targets M-cadherin in WB, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen M-cadherin fusion protein Ag25763 相同性解析による交差性が予測される生物種
Full Name cadherin 15, type 1, M-cadherin (myotubule)
Calculated molecular weight 89 kDa
Observed molecular weight130 kDa
GenBank accession numberBC008951
Gene Symbol M-cadherin
Gene ID (NCBI) 1013
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP55291
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. M-cadherin (muscle cadherin), also known as CDH15 (cadherin-15), is a 124-kDa type I transmembrane glycoprotein that is highly expressed in developing skeletal muscle, satellite cells, and cerebellum (PMID: 9545347; 12052883). It associates with β-catenin and functions as a cell-cell adhesion molecule in a homophilic and specific manner (PMID: 9545347). M-cadherin is part of the myogenic program and may provide a trigger for terminal muscle differentiation (PMID: 1840697).