Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue |
| Positive IHC detected in | Human Colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
10971-1-AP targets MAB21L2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | MAB21L2 fusion protein Ag1423 相同性解析による交差性が予測される生物種 |
| Full Name | mab-21-like 2 (C. elegans) |
| Calculated molecular weight | 41 kDa |
| Observed molecular weight | 41 kDa |
| GenBank accession number | BC009983 |
| Gene Symbol | MAB21L2 |
| Gene ID (NCBI) | 10586 |
| RRID | AB_2139234 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y586 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Mab-21 gene has been identified as a critical component required for sensory organ identity establishment in Caenorhabditis elegans. Mab21l1 and Mab21l2 paralogs have widespread and dynamic expression patterns during vertebrate development. Depletion of Mab21l1 and Mab21l2 messages in mouse embryo arrests axial turning, and impairs notochord and neural tube differentiation. Human MAB21L2 gene encodes mab-21-like 2 protein belonging to the mab-21 family. The Mab21l2-deficient mice have defects in eye and body wall formation, indicating that Mab21l2 plays crucial roles in retina and in ventral body wall formation. MAB21L2 plays a crucial role in both heart and liver development during mouse embryogenesis. This antibody is supposed to recognize both MAB21L2 and its homolog MAB21L1.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MAB21L2 antibody 10971-1-AP | Download protocol |
| IHC protocol for MAB21L2 antibody 10971-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancers (Basel) STAMP2 Expression Mediated by Cytokines Attenuates Their Growth-Limiting Effects in Prostate Cancer Cells. |