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  • KD/KO Validated

MACF1 Polyclonal antibody

MACF1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 13058-1-AP

Synonyms

MACF, KIAA0754, Actin cross-linking family protein 7, ACF7, ABP620


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Tested Applications

Positive WB detected inU-87 MG cells, NIH/3T3 cells, mouse lung tissue
Positive IHC detected inhuman lung tissue, human skeletal muscle tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU-251 cells, NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13058-1-AP targets MACF1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MACF1 fusion protein Ag3730 相同性解析による交差性が予測される生物種
Full Name microtubule-actin crosslinking factor 1
Calculated molecular weight 5430 aa, 620 kDa
Observed molecular weight600 kDa
GenBank accession numberBC007330
Gene symbol MACF1
Gene ID (NCBI) 23499
RRIDAB_2877907
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MACF1, also called ACF7 (actin crosslinking family 7), is a member of the spectraplakin family and is a ubiquitously expressed 614 kDa protein. This protein facilitates actin-microtubule interactions at the cell periphery and couples the microtubule network to cellular junctions. It displays a conserved role in membrane protrusion formation during evolution. The two major MACF1 isoforms characterized to date, MACF1a and MACF1b, result from alternative splicing. Both proteins contain N-terminal actin-binding domains (ABD), followed by plakin domains, extended spectrin repeat domains, EF-hand motifs, and C-terminal microtubule-binding domains (MTBDs). MACF1 is expressed in numerous tissue, including the skin, brain, skeletal muscle, and heart.

Protocols

Product Specific Protocols
WB protocol for MACF1 antibody 13058-1-APDownload protocol
IHC protocol for MACF1 antibody 13058-1-APDownload protocol
IF protocol for MACF1 antibody 13058-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Gastroenterology

Proteomic characterization identifies clinically relevant subgroups of gastrointestinal stromal tumors

Authors - Mingjun Sun
humanWB

Nat Commun

The genomic landscape of cholangiocarcinoma reveals the disruption of post-transcriptional modifiers.

Authors - Yaodong Zhang
mouseWB

Cell Death Differ

MACF1 promotes osteoblast differentiation by sequestering repressors in cytoplasm.

Authors - Lifang Hu
  • KD Validated
mouseWB

PLoS Genet

BBSome function is required for both the morphogenesis and maintenance of the photoreceptor outer segment.

Authors - Ying Hsu
humanWB

Oncotarget

LRP6 promotes invasion and metastasis of colorectal cancer through cytoskeleton dynamics.

Authors - Qian Yao
humanIHC

Biomed Pharmacother

Proteomics-based identification of tumor relevant proteins in lung adenocarcinoma.

Authors - Xin Zhou

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Joleen (Verified Customer) (06-03-2019)

MACF1 should be the top band but there were a lot of non specific bands as well.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1k
  • Cell Tissue Type: MDCK GII
MACF1 Antibody Western Blot, validation (1:1k dilution) in MDCK GII (Cat no:13058-1-AP)