Validation Data Gallery
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Tested Applications
| Positive WB detected in | Daudi cells, rat liver tissue, HEK-293 cells, HepG2 cells, Raji cells, THP-1 cells, HeLa cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | mouse ovary tissue, human liver cancer tissue, human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 20 publications below |
| IHC | See 6 publications below |
| IF | See 5 publications below |
Product Information
10337-1-AP targets MAD2L1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | MAD2L1 fusion protein Ag0307 相同性解析による交差性が予測される生物種 |
| Full Name | MAD2 mitotic arrest deficient-like 1 (yeast) |
| Calculated molecular weight | 24 kDa |
| Observed molecular weight | 25-30 kDa |
| GenBank accession number | BC000356 |
| Gene Symbol | MAD2L1 |
| Gene ID (NCBI) | 4085 |
| RRID | AB_2139365 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13257 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MAD2 mitotic arrest deficient-like 1 (yeast) (MAD2L1, synonyms: MAD2, HSMAD2) is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L1 is localized to human chromosome band 5q23.3 by in situ hybridization.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MAD2L1 antibody 10337-1-AP | Download protocol |
| IHC protocol for MAD2L1 antibody 10337-1-AP | Download protocol |
| IF protocol for MAD2L1 antibody 10337-1-AP | Download protocol |
| IP protocol for MAD2L1 antibody 10337-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Sci Adv The cohesin release factor Wapl interacts with Bub3 to govern SAC activity in female meiosis I. | ||
J Cell Biol Loss of centromeric RNA activates the spindle assembly checkpoint in mammalian female meiosis I. | ||
Oncogene KPNB1 inhibition disrupts proteostasis and triggers unfolded protein response-mediated apoptosis in glioblastoma cells. | ||
Cancer Res Deficiency of pRb family proteins and p53 in invasive urothelial tumorigenesis. | ||