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MAD2L1 Polyclonal antibody

Name: MAD2L1 Polyclonal antibody
Brand: Proteintech
SKU: 10337-1-AP

MAD2L1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 10337-1-AP

Synonyms

Mitotic arrest deficient 2-like protein 1, MAD2-like protein 1, MAD2 like protein 1, MAD2, HSMAD2

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis using 10337-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10337-1-AP (MAD2L1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis using 10337-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10337-1-AP (MAD2L1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 10337-1-AP
WB result of MAD2L1 antibody (10337-1-AP, 1:500) with si-control and si-MAD2L1 transfected HEK293 cells.
WB analysis of rat liver using 10337-1-AP
rat liver tissue were subjected to SDS PAGE followed by western blot with 10337-1-AP (MAD2L1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using 10337-1-AP
IP result of anti-MAD2L1 (IP:10337-1-AP, 3ug; Detection:10337-1-AP 1:500) with HEK-293 cells lysate 1800ug.
IHC staining of mouse ovary using 10337-1-AP
Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 10337-1-AP (MAD2L1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse ovary using 10337-1-AP
Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 10337-1-AP (MAD2L1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 10337-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer using 10337-1-AP (MAD2L1 antibody) at dilution of 1:50 (under 25x lens).
IHC staining of human ovary tumor using 10337-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10337-1-AP (MAD2L1 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human liver cancer using 10337-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer using 10337-1-AP (MAD2L1 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human liver cancer using 10337-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer using 10337-1-AP (MAD2L1 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human ovary tumor using 10337-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10337-1-AP (MAD2L1 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human ovary tumor using 10337-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10337-1-AP (MAD2L1 antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HepG2 using 10337-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MAD2L1 antibody (10337-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
IF Staining of HEK-293 using 10337-1-AP
Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using 10337-1-AP (MAD2L1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Cat No. 10337-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	Daudi cells, rat liver tissue, HEK-293 cells, HepG2 cells, Raji cells, THP-1 cells, HeLa cells
Positive IP detected in	HEK-293 cells
Positive IHC detected in	mouse ovary tissue, human liver cancer tissue, human ovary tumor tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells, HEK-293 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 18 publications below
IHC	See 6 publications below
IF	See 4 publications below

Product Information

10337-1-AP targets MAD2L1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	MAD2L1 fusion protein Ag0307 相同性解析による交差性が予測される生物種
Full Name	MAD2 mitotic arrest deficient-like 1 (yeast)
Calculated molecular weight	24 kDa
Observed molecular weight	25-30 kDa
GenBank accession number	BC000356
Gene symbol	MAD2L1
Gene ID (NCBI)	4085
RRID	AB_2139365
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

MAD2 mitotic arrest deficient-like 1 (yeast) (MAD2L1, synonyms: MAD2, HSMAD2) is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L1 is localized to human chromosome band 5q23.3 by in situ hybridization.

Protocols

Product Specific Protocols
WB protocol for MAD2L1 antibody 10337-1-AP	Download protocol
IHC protocol for MAD2L1 antibody 10337-1-AP	Download protocol
IF protocol for MAD2L1 antibody 10337-1-AP	Download protocol
IP protocol for MAD2L1 antibody 10337-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
	WB	Sci Adv The cohesin release factor Wapl interacts with Bub3 to govern SAC activity in female meiosis I. Authors - Changyin Zhou View Article
human	WB,IF	Nucleic Acids Res ULK1 phosphorylates Mad1 to regulate spindle assembly checkpoint. Authors - Fengjie Yuan View Article
	IF	J Cell Biol Loss of centromeric RNA activates the spindle assembly checkpoint in mammalian female meiosis I. Authors - Tianyu Wu View Article
human	WB	Oncogene KPNB1 inhibition disrupts proteostasis and triggers unfolded protein response-mediated apoptosis in glioblastoma cells. Authors - Zhi-Chuan Zhu View Article
	WB,IHC	Cancer Res Deficiency of pRb family proteins and p53 in invasive urothelial tumorigenesis. Authors - He Feng F View Article
		Cancer Res MPHOSPH1: a potential therapeutic target for hepatocellular carcinoma. Authors - Xinran Liu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MAD2L1 Polyclonal antibody

MAD2L1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis using 10337-1-AP

WB analysis using 10337-1-AP

WB analysis of HEK-293 using 10337-1-AP

WB analysis of rat liver using 10337-1-AP

IP experiment of HEK-293 using 10337-1-AP

IHC staining of mouse ovary using 10337-1-AP

IHC staining of mouse ovary using 10337-1-AP

IHC staining of human liver cancer using 10337-1-AP

IHC staining of human ovary tumor using 10337-1-AP

IHC staining of human liver cancer using 10337-1-AP

IHC staining of human liver cancer using 10337-1-AP

IHC staining of human ovary tumor using 10337-1-AP

IHC staining of human ovary tumor using 10337-1-AP

IF Staining of HepG2 using 10337-1-AP

IF Staining of HEK-293 using 10337-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

The cohesin release factor Wapl interacts with Bub3 to govern SAC activity in female meiosis I.

ULK1 phosphorylates Mad1 to regulate spindle assembly checkpoint.

Loss of centromeric RNA activates the spindle assembly checkpoint in mammalian female meiosis I.

KPNB1 inhibition disrupts proteostasis and triggers unfolded protein response-mediated apoptosis in glioblastoma cells.

Deficiency of pRb family proteins and p53 in invasive urothelial tumorigenesis.

MPHOSPH1: a potential therapeutic target for hepatocellular carcinoma.