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MAD2L1 Monoclonal antibody

MAD2L1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2F5F12

Cat no : 66014-1-Ig

Synonyms

HSMAD2, MAD2, MAD2 like protein 1, MAD2L1

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using MAD2L1 Monoclonal antibody (66014-1-Ig)

    WB analysis of HEK-293 using 66014-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66014-1-Ig (MAD2L1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using MAD2L1 Monoclonal antibody (66014-1-Ig)

    WB analysis of HEK-293 using 66014-1-Ig

    WB result of MAD2L1 antibody (66014-1-Ig, 1:2000) with si-control and si-MAD2L1 transfected HEK293 cells.

  • Western Blot (WB) analysis of Jurkat cells using MAD2L1 Monoclonal antibody (66014-1-Ig)

    WB analysis of Jurkat using 66014-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66014-1-Ig (MAD2L1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using MAD2L1 Monoclonal antibody (66014-1-Ig)

    WB analysis of COLO 320 using 66014-1-Ig

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 66014-1-Ig (MAD2L1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using MAD2L1 Monoclonal antibody (66014-1-Ig)

    WB analysis of HEK-293 using 66014-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66014-1-Ig (MAD2L1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using MAD2L1 Monoclonal antibody (66014-1-Ig)

    WB analysis of HeLa using 66014-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66014-1-Ig (MAD2L1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using MAD2L1 Monoclonal antibody (66014-1-Ig)

    IHC staining of human testis using 66014-1-Ig

    Immunohistochemical analysis of paraffin-embedded human testis slide using 66014-1-Ig (MAD2L1 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human testis tissue using MAD2L1 Monoclonal antibody (66014-1-Ig)

    IHC staining of human testis using 66014-1-Ig

    Immunohistochemical analysis of paraffin-embedded human testis slide using 66014-1-Ig (MAD2L1 Antibody) at dilution of 1:50.

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Tested Applications

Positive WB detected inHEK-293 cells, HEK-293
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 66014-1-Ig is MAD2L1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen MAD2L1 fusion protein Ag17512 相同性解析による交差性が予測される生物種
Full Name MAD2 mitotic arrest deficient-like 1 (yeast)
Calculated molecular weight 24 kDa
Observed molecular weight 24 kDa
GenBank accession numberBC000356
Gene symbol MAD2L1
Gene ID (NCBI) 4085
RRIDAB_11232424
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MAD2 mitotic arrest deficient-like 1 (yeast) (MAD2L1, synonyms: MAD2, HSMAD2) is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L1 is localized to human chromosome band 5q23.3 by in situ hybridization.

Protocols

Product Specific Protocols
WB protocol for MAD2L1 antibody 66014-1-IgDownload protocol
IHC protocol for MAD2L1 antibody 66014-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

FEBS Open Bio

SETDB2 interacts with BUBR1 to induce accurate chromosome segregation independently of its histone methyltransferase activity

Authors - Yanhong Tu
View Article