MAD2L1BP Polyclonal antibody
MAD2L1BP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 15344-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A431 cells, K-562 cells, HeLa cells, A549 cells, HepG2 cells |
Positive IHC detected in | human skin tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | A431 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
15344-1-AP targets MAD2L1BP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | MAD2L1BP fusion protein Ag7550 相同性解析による交差性が予測される生物種 |
Full Name | MAD2L1 binding protein |
Calculated molecular weight | 34 kDa |
Observed molecular weight | 34 kDa |
GenBank accession number | BC002904 |
Gene symbol | MAD2L1BP |
Gene ID (NCBI) | 9587 |
RRID | AB_10836940 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MAD2L1BP, also named as CMT2 and KIAA0110, belongs to the MAD2L1BP family. It may function to silence the spindle checkpoint and allow mitosis to proceed through anaphase by binding MAD2L1 after it has become dissociated from the MAD2L1-CDC20 complex. MAD2L1BP interacts with the spindle checkpoint and coordinates cell cycle events in late mitosis. MAD2L1BP may play an active role to remove MAD2 from p55CDC and tether MAD2 for silencing the checkpoint.
Protocols
Product Specific Protocols | |
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WB protocol for MAD2L1BP antibody 15344-1-AP | Download protocol |
IHC protocol for MAD2L1BP antibody 15344-1-AP | Download protocol |
IF protocol for MAD2L1BP antibody 15344-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |