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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MAD2L1BP Monoclonal antibody

MAD2L1BP Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2H3H1

Cat no : 68336-1-Ig

Synonyms

Caught by MAD2 protein, CMT2, KIAA0110, MAD2L1 binding protein, MAD2L1BP, RP1 261G23.6

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MAD2L1BP Monoclonal antibody (68336-1-Ig)

    WB analysis using 68336-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68336-1-Ig (MAD2L1BP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inA431 cells, HepG2 cells, MCF-7 cells, MDA-MB-468 cells, MDA-MB-231 cells, Jurkat cells, K-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68336-1-Ig targets MAD2L1BP in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen MAD2L1BP fusion protein Ag7653 相同性解析による交差性が予測される生物種
Full Name MAD2L1 binding protein
Calculated molecular weight 34 kDa
Observed molecular weight34 kDa
GenBank accession numberBC002904
Gene symbol MAD2L1BP
Gene ID (NCBI) 9587
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MAD2L1BP, also named as CMT2 and KIAA0110, belongs to the MAD2L1BP family. It may function to silence the spindle checkpoint and allow mitosis to proceed through anaphase by binding MAD2L1 after it has become dissociated from the MAD2L1-CDC20 complex. MAD2L1BP interacts with the spindle checkpoint and coordinates cell cycle events in late mitosis. MAD2L1BP may play an active role to remove MAD2 from p55CDC and tether MAD2 for silencing the checkpoint.

Protocols

Product Specific Protocols
WB protocol for MAD2L1BP antibody 68336-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols