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KD/KO Validated

MAD2L2 Polyclonal antibody

Name: MAD2L2 Polyclonal antibody
Brand: Proteintech
SKU: 12683-1-AP

MAD2L2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 12683-1-AP

Synonyms

hREV7, MAD2 like protein 2, MAD2B, MAD2L2, REV7, REV7 homolog

Validation Data Gallery

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AllWBIPIHC

WB analysis of human brain using 12683-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 12683-1-AP (MAD2L2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 12683-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 12683-1-AP (MAD2L2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 12683-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 12683-1-AP (MAD2L2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 12683-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 12683-1-AP (MAD2L2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 12683-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 12683-1-AP (MAD2L2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of fetal human brain using 12683-1-AP
fetal human brain tissue were subjected to SDS PAGE followed by western blot with 12683-1-AP (MAD2L2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of human kidney using 12683-1-AP
human kidney tissue were subjected to SDS PAGE followed by western blot with 12683-1-AP (MAD2L2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human colon using 12683-1-AP
human colon tissue were subjected to SDS PAGE followed by western blot with 12683-1-AP (MAD2L2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of A375 using 12683-1-AP
A375 cells were subjected to SDS PAGE followed by western blot with 12683-1-AP (MAD2L2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 12683-1-AP
IP result of anti-MAD2L2 (IP:12683-1-AP, 3ug; Detection:12683-1-AP 1:500) with mouse brain tissue lysate 3600ug.
IHC staining of human ovary tumor using 12683-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12683-1-AP (MAD2L2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary tumor using 12683-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12683-1-AP (MAD2L2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lymphoma using 12683-1-AP
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 12683-1-AP (MAD2L2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lymphoma using 12683-1-AP
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 12683-1-AP (MAD2L2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Cat No. 12683-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	human brain tissue, A375 cells, fetal HEK-293 cells, HeLa cells, human colon tissue, human kidney tissue, K-562 cells, mouse brain tissue
Positive IP detected in	mouse brain tissue
Positive IHC detected in	human ovary tumor tissue, human lymphoma tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2400
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 5 publications below
WB	See 15 publications below
IHC	See 2 publications below
IF	See 1 publications below
IP	See 1 publications below

Product Information

12683-1-AP targets MAD2L2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	MAD2L2 fusion protein Ag3373 相同性解析による交差性が予測される生物種
Full Name	MAD2 mitotic arrest deficient-like 2 (yeast)
Calculated molecular weight	211 aa, 24 kDa
Observed molecular weight	24 kDa
GenBank accession number	BC015244
Gene symbol	MAD2L2
Gene ID (NCBI)	10459
RRID	AB_2139530
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

MAD family, together with BUB and Mps1,Cdc20k, play roles in the mitotic spindle checkpoint. MAD2L2 is one of the MAD family. It can mediate the second polymerase switching in translation DNA synthesis by mediating the interaction between the error-prone DNA polymerase zeta catalytic subunit REV3L and the inserter polymerase REV1. Through regulation of the JNK-mediate phosphorylation and activation of the transcriptional activator ELK1, MAD2L2 involves in cellular response to DNA damage. Also it has role in the progression of cell cycle and peithelial-mesenchymal transdifferentiation.

Protocols

Product Specific Protocols
WB protocol for MAD2L2 antibody 12683-1-AP	Download protocol
IHC protocol for MAD2L2 antibody 12683-1-AP	Download protocol
IP protocol for MAD2L2 antibody 12683-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nat Commun MAD2L2 dimerization and TRIP13 control shieldin activity in DNA repair. Authors - Inge de Krijger KO Validated View Article
mouse	WB	Nat Commun SHLD1 is dispensable for 53BP1-dependent V(D)J recombination but critical for productive class switch recombination. Authors - Estelle Vincendeau View Article
human	WB, IP	J Cell Biol Sequestration of CDH1 by MAD2L2 prevents premature APC/C activation prior to anaphase onset. Authors - Tamar Listovsky KD Validated View Article
human	WB	Nucleic Acids Res REV7 is essential for DNA damage tolerance via two REV3L binding sites in mammalian DNA polymerase ζ Authors - Junya Tomida KD Validated View Article
human	WB	Nucleic Acids Res REV1 and polymerase ζ facilitate homologous recombination repair. Authors - Sharma Shilpy S KD Validated View Article
human	WB	Nucleic Acids Res Regulation of error-prone translesion synthesis by Spartan/C1orf124. Authors - Kim Myoung Shin MS View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MAD2L2 Polyclonal antibody

MAD2L2 Polyclonal Antibody for WB, IP, IHC, ELISA

Validation Data Gallery

WB analysis of human brain using 12683-1-AP

WB analysis of mouse brain using 12683-1-AP

WB analysis of HeLa using 12683-1-AP

WB analysis of HEK-293 using 12683-1-AP

WB analysis of K-562 using 12683-1-AP

WB analysis of fetal human brain using 12683-1-AP

WB analysis of human kidney using 12683-1-AP

WB analysis of human colon using 12683-1-AP

WB analysis of A375 using 12683-1-AP

IP experiment of mouse brain using 12683-1-AP

IHC staining of human ovary tumor using 12683-1-AP

IHC staining of human ovary tumor using 12683-1-AP

IHC staining of human lymphoma using 12683-1-AP

IHC staining of human lymphoma using 12683-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

MAD2L2 dimerization and TRIP13 control shieldin activity in DNA repair.

SHLD1 is dispensable for 53BP1-dependent V(D)J recombination but critical for productive class switch recombination.

Sequestration of CDH1 by MAD2L2 prevents premature APC/C activation prior to anaphase onset.

REV7 is essential for DNA damage tolerance via two REV3L binding sites in mammalian DNA polymerase ζ

REV1 and polymerase ζ facilitate homologous recombination repair.

Regulation of error-prone translesion synthesis by Spartan/C1orf124.

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