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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MAEA Recombinant antibody (83267-1-RR)

    WB analysis using 83267-1-RR (same clone as 83267-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83267-1-RR (MAEA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83267-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of U-251 cells using MAEA Recombinant antibody (83267-1-RR)

    FC experiment of U-251 using 83267-1-RR (same clone as 83267-1-PBS)

    1x10^6 U-251 cells were intracellularly stained with 0.25 ug MAEA Recombinant antibody (83267-1-RR, Clone:240097B2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83267-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00233-1

    Cytometric bead array standard curve of MP00233-1

    Cytometric bead array standard curve ofMP00233-1, MAEA Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83267-1-PBS. Detection antibody: 83267-3-PBS. Standard: Ag27150. Range: 2.5-100 ng/mL.

  • Cytometric bead array standard curve of MP00233-2

    Cytometric bead array standard curve of MP00233-2

    Cytometric bead array standard curve ofMP00233-2, MAEA Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83267-1-PBS. Detection antibody: 83267-2-PBS. Standard: Ag27150. Range: 1.25-100 ng/mL.

  • Affinity and Kinetic Characterization of 83267-1-RR

    Affinity and Kinetic Characterization of 83267-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83267-1-RR against Human MAEA were performed. The affinity constant is 0.496 nM.

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MAEA Recombinant antibody, PBS Only (Capture)

MAEA Uni-rAbTM Recombinant Antibody for Indirect ELISA, Cytometric bead array, WB
Cat No. 83267-1-PBS
Clone No.240097B2

Host / Isotype

Rabbit / IgG

Reactivity

mouse, human

Applications

Indirect ELISA, Cytometric bead array, WB

Synonyms

EMP, EMLP, EC:2.3.2.27, E3 ubiquitin-protein transferase MAEA, Cell proliferation-inducing gene 5 protein

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83267-1-PBS targets MAEA as part of a matched antibody pair:

MP00233-1: 83267-1-PBS capture and 83267-3-PBS detection (validated in Cytometric bead array)

MP00233-2: 83267-1-PBS capture and 83267-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity mouse, human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Affinity
KD<1.00 x 10-12M
KOff<1.00 x 10-6M
KOn=9.49 x 104M
Immunogen MAEA fusion protein Ag27150 相同性解析による交差性が予測される生物種
Full Name macrophage erythroblast attacher
Calculated molecular weight 45 kDa
GenBank accession numberBC001225
Gene Symbol MAEA
Gene ID (NCBI) 10296
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ7L5Y9
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.