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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MAGEA1 Polyclonal antibody

MAGEA1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12233-1-AP

Synonyms

Antigen MZ2 E, Cancer/testis antigen 1.1, CT1.1, MAGE 1 antigen, MAGE1, MAGE1A, MAGEA1, Melanoma associated antigen 1

Validation Data Gallery

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  • Western Blot (WB) analysis of A375 cells using MAGEA1 Polyclonal antibody (12233-1-AP)

    WB analysis of A375 using 12233-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 12233-1-AP (MAGEA1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using MAGEA1 Polyclonal antibody (12233-1-AP)

    IHC staining of human testis using 12233-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 12233-1-AP (MAGEA1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using MAGEA1 Polyclonal antibody (12233-1-AP)

    IHC staining of human testis using 12233-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 12233-1-AP (MAGEA1 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

Positive WB detected inA375 cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12233-1-AP targets MAGEA1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAGEA1 fusion protein Ag2871 相同性解析による交差性が予測される生物種
Full Name melanoma antigen family A, 1 (directs expression of antigen MZ2-E)
Calculated molecular weight 309 aa, 34 kDa
Observed molecular weight 43 kDa
GenBank accession numberBC017555
Gene symbol MAGEA1
Gene ID (NCBI) 4100
RRIDAB_2250080
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MAGEA1 antibody 12233-1-APDownload protocol
IHC protocol for MAGEA1 antibody 12233-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols