MAGEA1 Polyclonal antibody
MAGEA1 Polyclonal Antibody for WB, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 55105-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A375 cells, MCF-7 cells |
Positive IF/ICC detected in | A375 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
55105-1-AP targets MAGEA1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
Full Name | melanoma antigen family A, 1 (directs expression of antigen MZ2-E) |
Calculated molecular weight | 34 kDa |
Observed molecular weight | 46 kDa |
GenBank accession number | NM_004988 |
Gene symbol | MAGEA1 |
Gene ID (NCBI) | 4100 |
RRID | AB_10859942 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
Background Information
MAGEA1, also named as CT1.1, MZ2-E, MAGE1 and MAGE1A, is the first gene reported to encode a CTL-defined human tumor antigen. It may play a role in embryonal development and tumor transformation or aspects of tumor progression. Antigen recognized on a melanoma by autologous cytolytic T-lymphocytes. MAGEA1 provided direct evidence for the power of the cDNA expression library as an effective strategy to identify genes encoding tumor antigens recognized by HLA-restricted T cells.(PMID:16951217 ) The antibody is specific to MAGEA1.
Protocols
Product Specific Protocols | |
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WB protocol for MAGEA1 antibody 55105-1-AP | Download protocol |
IF protocol for MAGEA1 antibody 55105-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |