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Validation Data Gallery

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  • Western Blot (WB) analysis of A375 cells using MAGEA1 Polyclonal antibody (55105-1-AP)

    WB analysis of A375 using 55105-1-AP (same clone as 55105-1-PBS)

    A375 cells were subjected to SDS PAGE followed by western blot with 55105-1-AP (MAGEA1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55105-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using MAGEA1 Polyclonal antibody (55105-1-AP)

    WB analysis of MCF-7 using 55105-1-AP (same clone as 55105-1-PBS)

    MCF7 cells were subjected to SDS PAGE followed by western blot with 55105-1-AP (MAGEA1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55105-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A375 cells using MAGEA1 Polyclonal antibody (55105-1-AP)

    IF Staining of A375 using 55105-1-AP (same clone as 55105-1-PBS)

    Immunofluorescent analysis of A375 cells using 55105-1-AP (MAGEA1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 55105-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

55105-1-PBS targets MAGEA1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name melanoma antigen family A, 1 (directs expression of antigen MZ2-E)
Calculated molecular weight 34 kDa
Observed molecular weight 46 kDa
GenBank accession numberNM_004988
Gene Symbol MAGEA1
Gene ID (NCBI) 4100
RRIDAB_10859942
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP43355
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

MAGEA1, also named as CT1.1, MZ2-E, MAGE1 and MAGE1A, is the first gene reported to encode a CTL-defined human tumor antigen. It may play a role in embryonal development and tumor transformation or aspects of tumor progression. Antigen recognized on a melanoma by autologous cytolytic T-lymphocytes. MAGEA1 provided direct evidence for the power of the cDNA expression library as an effective strategy to identify genes encoding tumor antigens recognized by HLA-restricted T cells.(PMID:16951217 ) The antibody is specific to MAGEA1.