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Validation Data Gallery

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  • Western Blot (WB) analysis of A375 cells using MAGEA10 Polyclonal antibody (15295-1-AP)

    WB analysis of A375 using 15295-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 15295-1-AP (MAGEA10 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using MAGEA10 Polyclonal antibody (15295-1-AP)

    WB analysis of Jurkat using 15295-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 15295-1-AP (MAGEA10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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MAGEA10 Polyclonal antibody

MAGEA10 Polyclonal Antibody for WB, ELISA
Cat No. 15295-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Cancer/testis antigen 1.10, CT1.10, MAGE 10 antigen, MAGE10, MAGEA10, melanoma antigen family A, 10, Melanoma associated antigen 10

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA375 cells, Jurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

15295-1-AP targets MAGEA10 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAGEA10 fusion protein Ag7540 相同性解析による交差性が予測される生物種
Full Name melanoma antigen family A, 10
Calculated molecular weight 41 kDa
Observed molecular weight 62 kDa, 41 kDa
GenBank accession numberBC004105
Gene Symbol MAGEA10
Gene ID (NCBI) 4109
RRIDAB_2137968
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP43363
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MAGEA10 antibody 15295-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

BMC Cancer

Post-transcriptional regulation of cancer/testis antigen MAGEC2 expression by TRIM28 in tumor cells.

Authors - Xiao Song
View Article