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Validation Data Gallery

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  • Western Blot (WB) analysis of A375 cells using MAGEA11 Polyclonal antibody (15474-1-AP)

    WB analysis of A375 using 15474-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 15474-1-AP (MAGEA11 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A375 cells using MAGEA11 Polyclonal antibody (15474-1-AP)

    IF Staining of A375 using 15474-1-AP

    Immunofluorescent analysis of A375 cells using 15474-1-AP (MAGEA11 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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MAGEA11 Polyclonal antibody

MAGEA11 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 15474-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

Cancer/testis antigen 1.11, CT1.11, MAGE 11, MAGE 11 antigen, MAGE11, MAGEA 11, MAGEA11, melanoma antigen family A, 11, Melanoma associated antigen 11

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA375 cells
Positive IF/ICC detected inA375 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

15474-1-AP targets MAGEA11 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAGEA11 fusion protein Ag7778 相同性解析による交差性が予測される生物種
Full Name melanoma antigen family A, 11
Calculated molecular weight 48 kDa
Observed molecular weight 48 kDa, 44 kDa
GenBank accession numberBC004479
Gene Symbol MAGEA11
Gene ID (NCBI) 4110
RRIDAB_2137973
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP43364
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MAGEA11 antibody 15474-1-APDownload protocol
IF protocol for MAGEA11 antibody 15474-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Sci Rep

Integrated analysis of single-cell and bulk RNA-sequencing reveals a novel signature based on NK cell marker genes to predict prognosis and immunotherapy response in gastric cancer

Authors - Jian-Rong Sun
View Article